Mesenchymal Stem Cells - Emphasis in Adipose Tissue

被引:10
作者
Bertassoli, Bruno Machado [1 ]
de Assis Neto, Antonio Chaves [2 ]
de Oliveira, Franceliusa Delys [2 ]
Machado Arroyo, Maria Angelica [2 ]
Pires Ferrao, Juliana Shimara [2 ]
da Silva, Jodonai Barbosa [2 ]
Pignatari, Graciela Conceicao [2 ]
Braga, Patricia Beltrao [2 ]
机构
[1] Univ Fed Minas Gerais, Dept Morfol PPG Biol Celular, Inst Ciencias Biol, BR-31270901 Belo Horizonte, MG, Brazil
[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Cirurgia, PPG Anat Anim Domest & Silvestres, BR-05508270 Sao Paulo, Brazil
关键词
Adipose Tissue; Stem Cells; Mesenchymal Stem Cells; BONE-MARROW; STROMAL CELLS; DIFFERENTIATION; EXPRESSION; RABBIT; REPAIR;
D O I
10.1590/S1516-89132013000400011
中图分类号
Q [生物科学];
学科分类号
090105 [作物生产系统与生态工程];
摘要
The study of stem cells has evolved rapidly in recent decades. The importance is given to the concept that these cells are potentially able to become any cell type and have the power of self-renewal throughout the life of the organism. Mesenchymal stem cells (MSCs) can be isolated from various organs of the body such as bone marrow, adipose tissue, synovium, muscle and dermis, deciduous teeth, umbilical cord, placenta, liver, spleen and thymus. After their isolation in vitro, mesenchymal stem cells have the capacity to differentiate into various mesenchymal lineages and various tissues after the use of appropriate cultures. Studies have reported that mesenchymal stem cells from adipose tissue have the potential to differentiate themselves, like the cells commonly studied bone marrow. Adipose tissue is attractive due to its easy access, rapid expansion in vitro and only one collects the large amount of tissue. This review intends to show the protocols for isolation, cell culture and means of commercial cellular differentiation most widely used with emphasis on adipose tissue.
引用
收藏
页码:607 / 617
页数:11
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