Combined effects of initial sucrose concentration and inoculum size on cell growth and ginseng saponin production by suspension cultures of Panax ginseng

被引:54
作者
Akalezi, CO [1 ]
Liu, S [1 ]
Li, QS [1 ]
Yu, JT [1 ]
Zhong, JJ [1 ]
机构
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
关键词
ginseng saponin; initial sucrose concentration; inoculum size; Panax ginseng; plant cell culture; useful metabolite production;
D O I
10.1016/S0032-9592(98)00132-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cell growth and ginseng saponin production by suspension cultures of Panax ginseng were investigated under various initial sucrose concentrations and inoculum sizes. Cell growth was low at a low inoculum size of 1.5 g DW/1, and the maximum cell growth rate was obtained at 3 g DW/1 of inoculum size. A cell density of 22.4 g/l was obtained at inoculum size of 6 g DW/1 and initial sucrose concentration of 60 g/l after 26 days cultivation. The maximum cell yield of 0.83 was obtained at inoculum size of 3 g DW/1 and initial sucrose level of 30 g/l. Saponin biosynthesis was stimulated with high initial sucrose concentrations (60-80 g/l), and the maximum saponin production of 275 mg/l was achieved at 6 g/l of inoculum size and 60 g/l initial medium sucrose. This work is considered to be helpful for efficient large-scale bioprocessing of the ginseng cell cultures in bioreactors. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:639 / 642
页数:4
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