In vitro selection of RNA aptamers carrying multiple biotin groups in the side chains

被引：12

作者：

Ito, Y ^{[1
]}

Suzuki, A

Kawazoe, N

Imanishi, Y

机构：

[1] Univ Tokushima, Fac Engn, Dept Biol Sci & Technol, Tokushima 7708506, Japan

[2] Kyoto Univ, Grad Sch Engn, Dept Chem Mat, Kyoto 6068501, Japan

[3] NAIST, Grad Sch Mat Sci, Ikoma 6300101, Japan

来源：

BIOCONJUGATE CHEMISTRY | 2001年 / 12卷 / 06期

关键词：

D O I：

10.1021/bc0001342

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro selection or the systematic evolution of ligands by an exponential enrichment (SELEX) technique using a biotin-carrying nucleotide monomer was used for the development of a new type of molecular recognition sensor. Cytidine triphosphate (CTP) carrying the biotinyl group at the N-4-position was applied for the technique. A pool of random sequence RNAs containing biotinyl groups in the side chains was prepared, and the RNAs binding to adenosine 5'-triphosphate (ATP) were selected. The selected nonnatural RNAs were used for an assay of ATP. Since they carried multiple biotin groups in the side chains,. the sensitivity was high.

引用

页码：850 / 854

页数：5

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