Efficient Gene Delivery Vectors by Tuning the Surface Charge Density of Amino Acid-Functionalized Gold Nanoparticles

被引：369

作者：

Ghosh, Partha S. ^{[1
]}

Kim, Chae-Kyu ^{[1
]}

Han, Gang ^{[1
]}

Forbes, Neil S. ^{[2
]}

Rotello, Vincent M. ^{[1
]}

机构：

[1] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA

[2] Univ Massachusetts, Dept Chem Engn, Amherst, MA 01003 USA

来源：

ACS NANO | 2008年 / 2卷 / 11期

基金：

美国国家科学基金会;

关键词：

gold nanoparticles; transfection; glutathione; delivery; DNA;

D O I：

10.1021/nn800507t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Gold colloids functionalized with amino acids provide a scaffold for effective DNA binding with subsequent condensation. Particles with lysine and lysine dendron functionality formed particularly compact complexes and provided highly efficient gene delivery without any observed cytotoxicity. Nanoparticles functionalized with first generation lysine dendrons (NP-LysG1) were similar to 28-fold superior to polylysine in reporter gene expression. These amino acid-based nanoparticles were responsive to intracellular glutathione levels, providing a tool for controlled release and concomitant expression of DNA.

引用

页码：2213 / 2218

页数：6

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