Transforming growth factor-beta and hepatocyte growth factor produced by gastric fibroblasts stimulate the invasiveness of scirrhous gastric cancer cells

被引：79

作者：

Inoue, T ^{[1
]}

Chung, YS ^{[1
]}

Yashiro, M ^{[1
]}

Nishimura, S ^{[1
]}

Hasuma, T ^{[1
]}

Otani, S ^{[1
]}

Sowa, M ^{[1
]}

机构：

[1] OSAKA CITY UNIV,SCH MED,DEPT BIOCHEM 2,ABENO KU,OSAKA 545,JAPAN

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1997年 / 88卷 / 02期

关键词：

scirrhous gastric carcinoma; fibroblast; invasion; transforming growth factor-beta; hepatocyte growth factor;

D O I：

10.1111/j.1349-7006.1997.tb00360.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Scirrhous gastric carcinoma is characterized by cancer cells that infiltrate rapidly in the stroma with extensive growth of fibroblasts. In the present study, we examined the effect of gastric fibroblasts on the invasiveness of a scirrhous gastric cancer cell line, OCUM-2D, using an invasion assay. Gastric fibroblast-derived conditioned medium (CR I) significantly stimulated the invasiveness of OCUM-2D cells, as did transforming growth factor-beta (TGF-beta) and hepatocyte growth factor (I-IGF). The stimulating activity of gastric fibroblast-derived CM was inhibited significantly by anti-TGF-beta neutralizing antibody or anti-HGF neutralizing antibody. TGF-beta and HGF Here detected in the gastric fibroblast-derived Chi, and TGF-beta receptor and C-met (HGF receptor) were expressed on OCUM-2D cells. Thus, TGF-beta and HGF produced by gastric fibroblasts appear to affect the invasiveness of scirrhous gastric cancer cells. TGF-beta was also detected in the conditioned medium derived from OCUM-2D cells, though HGF was not. TGF-beta appears to affect the invasiveness of OCUM-2D cells in both paracrine and autocrine fashions.

引用

页码：152 / 159

页数：8

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