Hemoxygenase and nitric oxide synthase do not maintain human uterine quiescence during pregnancy

被引:25
作者
Barber, A
Robson, SC
Lyall, F [1 ]
机构
[1] Duncan Guthrie Inst Med Genet, Maternal & Fetal Med Sect, Glasgow G3 8SJ, Lanark, Scotland
[2] Royal Victoria Infirm, Dept Obstet & Gynaecol, Newcastle Upon Tyne NE1 4LP, Tyne & Wear, England
关键词
D O I
10.1016/S0002-9440(10)65182-6
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
The nitric oxide (NO)-cGMP pathway has been proposed as a mechanism for relaxation of myometrium during pregnancy and as a modulator of labor. Carbon monoxide (CO), produced by hemeoxygenases (HO-1 and HO-2), also activates soluble guanylate cyclase to increase cGMP, A recent study reported a large increase in HO-1 and HO-2 proteins during pregnancy, suggesting that the MO-CO pathway may be important in the maintenance of uterine quiescence during pregnancy. In this study we used Western blotting, reverse transcription-polymerase chain reaction, and immunohistochemistry to determine HO-1 and HO-2 expression in nonpregnant, pregnant, and laboring myometrium, Immunolocalization of HO was also compared with endothelial and inducible nitric oxide synthases (eNOS and iNOS). In contrast to HO-1 protein, which was not detected in myometrium, HO-2 protein and mRNA were constitutively expressed, although there were no differences in expression between the groups. eNOS was expressed in endothelial cells but not in myometrial. smooth muscle. iNOS protein was not detected in myometrium, These data do not support an up-regulation of HO-1 and HO-2 during pregnancy and are not consistent with a role for NO of a major role for CO in human. myometrial quiescence. Our results are also in keeping with HO-2 being an noninducible protein.
引用
收藏
页码:831 / 840
页数:10
相关论文
共 47 条