Isolation and purification of lutein from the microalga Chlorella vulgaris by extraction after saponification

被引：69

作者：

Li, HB ^{[1
]}

Jiang, Y ^{[1
]}

Chen, F ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2002年 / 50卷 / 05期

关键词：

lutein; purification; Chlorella vulgaris; saponification; extraction;

D O I：

10.1021/jf010220b

中图分类号：

S [农业科学];

学科分类号：

09 [农学];

摘要：

A simple and efficient method for the isolation and purification of lutein from the microalga Chlorella vulgaris was developed. Crude lutein was obtained by extraction with dichloromethane from the microalga after saponification. Partition values of lutein in the two-phase system of ethanol-water-dichloromethane at different ratios were measured by HPLC so as to assist the determination of an appropriate condition for washing water-soluble impurities in the crude lutein. Partition values of lutein in another two-phase system of ethanol-water-hexane at different ratios were also measured by HPLC for determining the condition for removing fat-soluble impurities. The water-soluble impurities in the crude lutein were removed by washing with 30% aqueous ethanol, and the fat-soluble impurities were removed by extraction with hexane. The final purity of lutein obtained was 90-98%, and the yield was 85-91%.

引用

页码：1070 / 1072

页数：3

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