Supramolecular Host-Guest Pseudocomb Conjugates Composed of Multiple Star Polycations Tied Tunably with a Linear Polycation Backbone for Gene Transfection

被引：46

作者：

Hu, Y. ^{[1
]}

Chai, M. Y. ^{[1
]}

Yang, W. T. ^{[1
]}

Xu, F. J. ^{[1
]}

机构：

[1] Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Key Lab Carbon Fiber & Funct Polymers, Minist Educ,Coll Mat Sci & Engn, Beijing 100029, Peoples R China

来源：

BIOCONJUGATE CHEMISTRY | 2013年 / 24卷 / 06期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

TRANSFER RADICAL POLYMERIZATION; BETA-CYCLODEXTRIN CORES; POLY(GLYCIDYL METHACRYLATE); POLYMERS; COPOLYMER;

D O I：

10.1021/bc400115e

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

A series of novel supramolecular pseudocomb polycations (l-PGEA-Ad/CD-PGEAs) were synthesized by tying multiple low-molecular-weight beta-cyclodextrin (CD)-cored, ethanolamine-functionalized poly(glycidyl methacrylate) (PGEA) star polymers (CD-PGEAs) with an adamantine-modified linear PGEA (l-PGEA-Ad) backbone via the host guest interaction. The pseudocomb carriers were studied in terms of their DNA binding capabilities, cytotoxicities, and gene transfection efficiencies in the HepG2 and HEK293 cell lines. The pseudocomb l-PGEA-Ad/CD-PGEAs exhibited better plasmid DNA-condensing abilities than their counterparts, CD-PGEA and l-PGEA. Meanwhile, the pseudocomb carriers displayed low cytotoxicity, similar to CD-PGEA and l-PGEA. Moreover, the gene transfection efficiencies of the pseudocomb carriers were much higher than those of CD-PGEA and l-PGEA at various PGEA nitrogen/DNA phosphate molar ratios. Such supramolecular preparation of pseudocomb gene carriers could provide a flexible approach for adjusting the structure and functionality of supramolecular polymers via the proper use of non-covalent interactions.

引用

页码：1049 / 1056

页数：8

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