Transverse-relaxation-optimized (TROSY) gradient-enhanced triple-resonance NMR spectroscopy

被引：85

作者：

Loria, JP

Rance, M

Palmer, AG

机构：

[1] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA

[2] Univ Cincinnati, Dept Mol Genet Biochem & Microbiol, Cincinnati, OH 45267 USA

来源：

JOURNAL OF MAGNETIC RESONANCE | 1999年 / 141卷 / 01期

关键词：

TROSY; triple-resonance NMR spectroscopy; triosephosphate isomerase; gradient coherence selection;

D O I：

10.1006/jmre.1999.1891

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two modifications to sensitivity-enhanced gradient-selected TROSY-based triple-resonance NMR experiments are proposed that reduce the overall duration of the pulse sequences and minimize radiation damping effects on water-flipback solvent suppression. The modifications are illustrated for the HNCO-TROSY experiment, but are applicable to all triple-resonance experiments that detect proton magnetization after a reverse polarization transfer step from a N-15 spin. The methods are applied to yeast triosephosphate isomerase, a symmetric dimer with 248 amino acid residues per monomer, (C) 1999 Academic Press.

引用

收藏

页码：180 / 184

页数：5

相关论文

共 26 条

[11] IMPROVED SOLVENT SUPPRESSION IN ONE-DIMENSIONAL AND TWO-DIMENSIONAL NMR-SPECTRA BY CONVOLUTION OF TIME-DOMAIN DATA [J].

MARION, D ;

IKURA, M ;

BAX, A .

JOURNAL OF MAGNETIC RESONANCE, 1989, 84 (02) :425-430

[12] RAPID RECORDING OF 2D NMR-SPECTRA WITHOUT PHASE CYCLING - APPLICATION TO THE STUDY OF HYDROGEN-EXCHANGE IN PROTEINS [J].

MARION, D ;

IKURA, M ;

TSCHUDIN, R ;

BAX, A .

JOURNAL OF MAGNETIC RESONANCE, 1989, 85 (02) :393-399

[13] IMPROVED SENSITIVITY OF HSQC SPECTRA OF EXCHANGING PROTONS AT SHORT INTERSCAN DELAYS USING A NEW FAST HSQC (FHSQC) DETECTION SCHEME THAT AVOIDS WATER SATURATION [J].

MORI, S ;

ABEYGUNAWARDANA, C ;

JOHNSON, MO ;

VANZIJL, PCM .

JOURNAL OF MAGNETIC RESONANCE SERIES B, 1995, 108 (01) :94-98

[14] GRADIENT-ENHANCED TRIPLE-RESONANCE 3-DIMENSIONAL NMR EXPERIMENTS WITH IMPROVED SENSITIVITY [J].

MUHANDIRAM, DR ;

KAY, LE .

JOURNAL OF MAGNETIC RESONANCE SERIES B, 1994, 103 (03) :203-216

[15] SENSITIVITY IMPROVEMENT IN PROTON-DETECTED 2-DIMENSIONAL HETERONUCLEAR CORRELATION NMR-SPECTROSCOPY [J].

PALMER, AG ;

CAVANAGH, J ;

WRIGHT, PE ;

RANCE, M .

JOURNAL OF MAGNETIC RESONANCE, 1991, 93 (01) :151-170

[16] Attenuated T-2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution [J].

Pervushin, K ;

Riek, R ;

Wider, G ;

Wuthrich, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (23) :12366-12371

[17] Transverse relaxation-optimized spectroscopy (TROSY) for NMR studies of aromatic spin systems in 13C-labeled proteins [J].

Pervushin, K ;

Riek, R ;

Wider, G ;

Wüthrich, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (25) :6394-6400

[18] Sensitivity improvement of transverse relaxation-optimized spectroscopy [J].

Rance, M ;

Loria, JP ;

Palmer, AG .

JOURNAL OF MAGNETIC RESONANCE, 1999, 136 (01) :92-101

[19] TROSY-type triple-resonance experiments for sequential NMR assignments of large proteins [J].

Salzmann, M ;

Wider, G ;

Pervushin, K ;

Senn, H ;

Wüthrich, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (04) :844-848

[20] TROSY in triple-resonance experiments:: New perspectives for sequential NMR assignment of large proteins [J].

Salzmann, M ;

Pervushin, K ;

Wider, G ;

Senn, H ;

Wüthrich, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (23) :13585-13590