G Protein-Coupled Receptor-Type G Proteins Are Required for Light-Dependent Seedling Growth and Fertility in Arabidopsis

被引:36
作者
Jaffe, Felix W. [1 ]
Freschet, Gian-Enrico C. [1 ]
Valdes, Billy M. [1 ]
Runions, John [2 ]
Terry, Matthew J. [1 ]
Williams, Lorraine E. [1 ]
机构
[1] Univ Southampton, Ctr Biol Sci, Southampton SO17 1BJ, Hants, England
[2] Oxford Brookes Univ, Dept Biol & Med Sci, Oxford OX3 0BP, England
基金
英国生物技术与生命科学研究理事会;
关键词
HETEROTRIMERIC G-PROTEIN; HYPOCOTYL ELONGATION; TRANSCRIPTION FACTOR; FUNCTIONAL-ANALYSIS; ALPHA-SUBUNIT; ABA RECEPTORS; BETA-SUBUNIT; AUXIN; GENE; GERMINATION;
D O I
10.1105/tpc.112.098681
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
G protein-coupled receptor-type G proteins (GTGs) are highly conserved membrane proteins in plants, animals, and fungi that have eight to nine predicted transmembrane domains. They have been classified as G protein-coupled receptor-type G proteins that function as abscisic acid (ABA) receptors in Arabidopsis thaliana. We cloned Arabidopsis GTG1 and GTG2 and isolated new T-DNA insertion alleles of GTG1 and GTG2 in both Wassilewskija and Columbia backgrounds. These gtg1 gtg2 double mutants show defects in fertility, hypocotyl and root growth, and responses to light and sugars. Histological studies of shoot tissue reveal cellular distortions that are particularly evident in the epidermal layer. Stable expression of GTG1(pro):GTG1-GFP (for green fluorescent protein) in Arabidopsis and transient expression in tobacco (Nicotiana tabacum) indicate that GTG1 is localized primarily to Golgi bodies and to the endoplasmic reticulum. Microarray analysis comparing gene expression profiles in the wild type and double mutant revealed differences in expression of genes important for cell wall function, hormone response, and amino acid metabolism. The double mutants isolated here respond normally to ABA in seed germination assays, root growth inhibition, and gene expression analysis. These results are inconsistent with their proposed role as ABA receptors but demonstrate that GTGs are fundamentally important for plant growth and development.
引用
收藏
页码:3649 / 3668
页数:20
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