G protein-coupled receptor-type G proteins (GTGs) are highly conserved membrane proteins in plants, animals, and fungi that have eight to nine predicted transmembrane domains. They have been classified as G protein-coupled receptor-type G proteins that function as abscisic acid (ABA) receptors in Arabidopsis thaliana. We cloned Arabidopsis GTG1 and GTG2 and isolated new T-DNA insertion alleles of GTG1 and GTG2 in both Wassilewskija and Columbia backgrounds. These gtg1 gtg2 double mutants show defects in fertility, hypocotyl and root growth, and responses to light and sugars. Histological studies of shoot tissue reveal cellular distortions that are particularly evident in the epidermal layer. Stable expression of GTG1(pro):GTG1-GFP (for green fluorescent protein) in Arabidopsis and transient expression in tobacco (Nicotiana tabacum) indicate that GTG1 is localized primarily to Golgi bodies and to the endoplasmic reticulum. Microarray analysis comparing gene expression profiles in the wild type and double mutant revealed differences in expression of genes important for cell wall function, hormone response, and amino acid metabolism. The double mutants isolated here respond normally to ABA in seed germination assays, root growth inhibition, and gene expression analysis. These results are inconsistent with their proposed role as ABA receptors but demonstrate that GTGs are fundamentally important for plant growth and development.
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Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Japan Int Res Ctr Agr Sci, Biol Resources & Postharvest Div, Tsukuba, Ibaraki 3058686, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Kim, June-Sik
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Mizoi, Junya
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Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Mizoi, Junya
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Yoshida, Takuya
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Fujita, Yasunari
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Nakajima, Jun
论文数: 0引用数: 0
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Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Nakajima, Jun
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Ohori, Teppei
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Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Ohori, Teppei
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Todaka, Daisuke
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Japan Int Res Ctr Agr Sci, Biol Resources & Postharvest Div, Tsukuba, Ibaraki 3058686, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Todaka, Daisuke
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Nakashima, Kazuo
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Japan Int Res Ctr Agr Sci, Biol Resources & Postharvest Div, Tsukuba, Ibaraki 3058686, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Nakashima, Kazuo
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Hirayama, Takashi
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Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama 7100046, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Hirayama, Takashi
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Shinozaki, Kazuo
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RIKEN, Plant Sci Ctr, Yokohama, Kanagawa 2300045, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Shinozaki, Kazuo
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Yamaguchi-Shinozaki, Kazuko
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Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Japan Int Res Ctr Agr Sci, Biol Resources & Postharvest Div, Tsukuba, Ibaraki 3058686, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
机构:
4700 King Abdullah Univ Sci & Technol, Plant Stress Genom Res Ctr, Thuwal 239556900, Saudi Arabia
Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA4700 King Abdullah Univ Sci & Technol, Plant Stress Genom Res Ctr, Thuwal 239556900, Saudi Arabia
Klingler, John P.
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Batelli, Giorgia
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4700 King Abdullah Univ Sci & Technol, Plant Stress Genom Res Ctr, Thuwal 239556900, Saudi Arabia
Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA4700 King Abdullah Univ Sci & Technol, Plant Stress Genom Res Ctr, Thuwal 239556900, Saudi Arabia
Batelli, Giorgia
;
Zhu, Jian-Kang
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机构:
4700 King Abdullah Univ Sci & Technol, Plant Stress Genom Res Ctr, Thuwal 239556900, Saudi Arabia
Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA4700 King Abdullah Univ Sci & Technol, Plant Stress Genom Res Ctr, Thuwal 239556900, Saudi Arabia
机构:
Univ N Carolina, Dept Biol, Chapel Hill, NC USA
Univ N Carolina, Dept Pharmacol, Chapel Hill, NC USAUniv Cologne, Inst Bot, D-5000 Cologne, Germany
机构:
Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Japan Int Res Ctr Agr Sci, Biol Resources & Postharvest Div, Tsukuba, Ibaraki 3058686, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Kim, June-Sik
;
Mizoi, Junya
论文数: 0引用数: 0
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机构:
Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Mizoi, Junya
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机构:
Yoshida, Takuya
;
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机构:
Fujita, Yasunari
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Nakajima, Jun
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Nakajima, Jun
;
Ohori, Teppei
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Ohori, Teppei
;
Todaka, Daisuke
论文数: 0引用数: 0
h-index: 0
机构:
Japan Int Res Ctr Agr Sci, Biol Resources & Postharvest Div, Tsukuba, Ibaraki 3058686, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Todaka, Daisuke
;
Nakashima, Kazuo
论文数: 0引用数: 0
h-index: 0
机构:
Japan Int Res Ctr Agr Sci, Biol Resources & Postharvest Div, Tsukuba, Ibaraki 3058686, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Nakashima, Kazuo
;
Hirayama, Takashi
论文数: 0引用数: 0
h-index: 0
机构:
Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama 7100046, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Hirayama, Takashi
;
Shinozaki, Kazuo
论文数: 0引用数: 0
h-index: 0
机构:
RIKEN, Plant Sci Ctr, Yokohama, Kanagawa 2300045, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Shinozaki, Kazuo
;
Yamaguchi-Shinozaki, Kazuko
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
Japan Int Res Ctr Agr Sci, Biol Resources & Postharvest Div, Tsukuba, Ibaraki 3058686, JapanUniv Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
机构:
4700 King Abdullah Univ Sci & Technol, Plant Stress Genom Res Ctr, Thuwal 239556900, Saudi Arabia
Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA4700 King Abdullah Univ Sci & Technol, Plant Stress Genom Res Ctr, Thuwal 239556900, Saudi Arabia
Klingler, John P.
;
Batelli, Giorgia
论文数: 0引用数: 0
h-index: 0
机构:
4700 King Abdullah Univ Sci & Technol, Plant Stress Genom Res Ctr, Thuwal 239556900, Saudi Arabia
Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA4700 King Abdullah Univ Sci & Technol, Plant Stress Genom Res Ctr, Thuwal 239556900, Saudi Arabia
Batelli, Giorgia
;
Zhu, Jian-Kang
论文数: 0引用数: 0
h-index: 0
机构:
4700 King Abdullah Univ Sci & Technol, Plant Stress Genom Res Ctr, Thuwal 239556900, Saudi Arabia
Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA4700 King Abdullah Univ Sci & Technol, Plant Stress Genom Res Ctr, Thuwal 239556900, Saudi Arabia
机构:
Univ N Carolina, Dept Biol, Chapel Hill, NC USA
Univ N Carolina, Dept Pharmacol, Chapel Hill, NC USAUniv Cologne, Inst Bot, D-5000 Cologne, Germany