Catalytic hydrogenation of itaconic acid in a biotinylated Pyrphos-rhodium(I) system in a protein cavity

被引：93

作者：

Lin, CC

Lin, CW

Chan, ASC ^{[1
]}

机构：

[1] Hong Kong Polytech Univ, Open Lab Chirotechnol, Hong Kong, Peoples R China

[2] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Hong Kong, Peoples R China

来源：

TETRAHEDRON-ASYMMETRY | 1999年 / 10卷 / 10期

关键词：

D O I：

10.1016/S0957-4166(99)00193-7

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The construction of a chiral catalyst system embedded at a specific site in a protein has been studied. The preparation of the biotinylated Pyrphos-Rh(I) complex attached to the binding site in avidin and its application to the asymmetric hydrogenation of itaconic acid have been investigated. By introducing the chiral Pyrphos-Rh(I) moiety into the constrained environment of the protein cavity it was found that the enantioselectivity of the system was significantly influenced by the tertiary conformation within the avidin cavity. The effects of reaction conditions such as temperature, hydrogen pressure, and the pH value of the buffer on enantioselectivity are reported. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：1887 / 1893

页数：7

共 30 条

[21] A NEW ENZYME CONTAINING A SYNTHETICALLY FORMED ACTIVE SITE . THIOL-SUBTILLISIN [J].