Annotating genomes with massive-scale RNA sequencing

被引:172
作者
Denoeud, France [1 ,2 ,3 ]
Aury, Jean-Marc [1 ,2 ,3 ]
Da Silva, Corinne [1 ,2 ,3 ]
Noel, Benjamin [1 ,2 ,3 ]
Rogier, Odile [1 ,2 ,3 ]
Delledonne, Massimo [4 ]
Morgante, Michele [5 ]
Valle, Giorgio [6 ]
Wincker, Patrick [1 ,2 ,3 ]
Scarpelli, Claude [1 ,2 ,3 ]
Jaillon, Olivier [1 ,2 ,3 ]
Artiguenave, Francois [1 ,2 ,3 ]
机构
[1] Inst Genom, DSV, CEA, F-91057 Evry, France
[2] CNRS, UMR 8030, F-91057 Evry, France
[3] Univ Evry, F-91057 Evry, France
[4] Sci & Technol Dept, I-37134 Verona, Italy
[5] Ist Genom Applicata, I-33100 Udine, Italy
[6] Univ Padua, CRIBI, I-35121 Padua, Italy
关键词
D O I
10.1186/gb-2008-9-12-r175
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty of aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.
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收藏
页数:12
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