The wheat VRN2 gene is a flowering repressor down-regulated by vernalization

被引:830
作者
Yan, LL
Loukoianov, A
Blechl, A
Tranquilli, G
Ramakrishna, W
SanMiguel, P
Bennetzen, JL
Echenique, V
Dubcovsky, J [1 ]
机构
[1] Univ Calif Davis, Dept Agron & Range Sci, Davis, CA 95616 USA
[2] ARS, USDA, Albany, CA 94710 USA
[3] Michigan Technol Univ, Dept Biol Sci, Houghton, MI 49931 USA
[4] Purdue Univ, Purdue Univ Genom Core, W Lafayette, IN 47907 USA
[5] Univ Georgia, Dept Genet, Athens, GA 30602 USA
关键词
D O I
10.1126/science.1094305
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. We report here the positional cloning of the wheat vernalization gene VRN2, a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN2, whether by natural mutations or deletions, resulted in spring lines, which do not require vernalization to flower. Reduction of the RNA level of VRN2 by RNA interference accelerated the flowering time of transgenic winter-wheat plants by more than a month.
引用
收藏
页码:1640 / 1644
页数:5
相关论文
共 21 条
  • [11] Construction and characterization of a bacterial artificial chromosome (BAC) library for the A genome of wheat
    Lijavetzky, D
    Muzzi, G
    Wicker, T
    Keller, B
    Wing, R
    Dubcovsky, J
    [J]. GENOME, 1999, 42 (06) : 1176 - 1182
  • [12] FLOWERING LOCUS C encodes a novel MADS domain protein that acts as a repressor of flowering
    Michaels, SD
    Amasino, RM
    [J]. PLANT CELL, 1999, 11 (05) : 949 - 956
  • [13] THE CONSTANS GENE OF ARABIDOPSIS PROMOTES FLOWERING AND ENCODES A PROTEIN SHOWING SIMILARITIES TO ZINC-FINGER TRANSCRIPTION FACTORS
    PUTTERILL, J
    ROBSON, F
    LEE, K
    SIMON, R
    COUPLAND, G
    [J]. CELL, 1995, 80 (06) : 847 - 857
  • [14] Functional importance of conserved domains in the flowering-time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants
    Robson, F
    Costa, MMR
    Hepworth, SR
    Vizir, I
    Piñeiro, M
    Reeves, PH
    Putterill, J
    Coupland, G
    [J]. PLANT JOURNAL, 2001, 28 (06) : 619 - 631
  • [15] The FLF MADS box gene:: A repressor of flowering in Arabidopsis regulated by vernalization and methylation
    Sheldon, CC
    Burn, JE
    Perez, PP
    Metzger, J
    Edwards, JA
    Peacock, WJ
    Dennis, ES
    [J]. PLANT CELL, 1999, 11 (03) : 445 - 458
  • [16] Vernalization in Arabidopsis thaliana is mediated by the PHD finger protein VIN3
    Sung, SB
    Amasino, RM
    [J]. NATURE, 2004, 427 (6970) : 159 - 164
  • [17] TAKAHASHI R, 1971, P 2 INT BARL GEN S W, P388
  • [18] Takahashi R., 1983, Catalogue of barley germplasm preserved in Okayama University
  • [19] Epistatic interaction between vernalization genes Vrn-Am1 and Vrn-Am2 in diploid wheat
    Tranquilli, G
    Dubcovsky, J
    [J]. JOURNAL OF HEREDITY, 2000, 91 (04) : 304 - 306
  • [20] Positional cloning of the wheat vernalization gene VRN1
    Yan, L
    Loukoianov, A
    Tranquilli, G
    Helguera, M
    Fahima, T
    Dubcovsky, J
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (10) : 6263 - 6268