Efficient microRNA capture and bar-coding via enzymatic oligonucleotide adenylation

被引：31

作者：

Vigneault, Francois ^{[1
]}

Sismour, A. Michael ^{[1
]}

Church, George M. ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 09期

基金：

美国国家卫生研究院; 加拿大健康研究院;

关键词：

D O I：

10.1038/NMETH.1244

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we report a highly efficient and simplified strategy to preadenylate bar-coded oligonucleotides designed for microRNA (miRNA) capture and multiplex analysis. Using this approach, we enzymatically preadenylated bar-coded oligonucleotides with high efficiency when compared to the chemical method currently used by miRNA investigators. As a case study, we used these oligonucleotides in an ATP-independent ligation to miRNAs, suggesting the utility of our method in end-capture protocols and high-throughput sequencing applications.

引用

页码：777 / 779

页数：3

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