Studies on neurosteroids IX. Characterization of estrogens in rat brains using gas chromatography-tandem mass spectrometry

被引:13
作者
Shimada, K [1 ]
Mitamura, K [1 ]
Shiroyama, M [1 ]
Yago, K [1 ]
机构
[1] Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 9200934, Japan
关键词
steroids; neurosteroids; estrogens;
D O I
10.1016/S0021-9673(98)01083-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The characterization of the classical estrogens (estrone, estradiol, estriol) and guaiacol estrogens (2-hydroxyestrone 3-methyl ether, 4-hydroxyestrone 3-methyl ether) in rat brains was performed using gas chromatography-tandem mass spectrometry (GC-MS-MS). Estrogens were purified from Wistar strain rat brains by some chromatographic pre-treatments, such as solid-phase extraction, preparative thin-layer chromatography or preparative high-performance liquid chromatography. After the derivatization with O-methylhydroxylamine and/or N,O-bis(trimethylsilyl)trifluoroacetamide estrogens were identified by comparison of their chromatographic behavior during GC-MS-MS operating in the product ion scan mode and comparison with the product ion MS spectra of an authentic sample. These evidences suggested that estrogens exist in rat brains as neurosteroids or neuroactive steroids. (C) 1999 Elsevier Science BN. All rights reserved.
引用
收藏
页码:171 / 178
页数:8
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