Characterization of the endonuclease SSO2001 from Sulfolobus solfataricus P2

被引：53

作者：

Han, Dong ^{[1
]}

Krauss, Gerhard ^{[1
]}

机构：

[1] Univ Bayreuth, Dept Biochem, D-95447 Bayreuth, Germany

来源：

FEBS LETTERS | 2009年 / 583卷 / 04期

关键词：

CRISPR-associated; Clustered regularly interspaced short; palindromic repeats; Nuclease; SSO2001; ALICYCLOBACILLUS-ACIDOCALDARIUS; AFFINITY TAG; HD DOMAIN; PROTEIN; PROKARYOTES; REPEATS; GENES; CHAPERONIN; REPORTER; ELEMENTS;

D O I：

10.1016/j.febslet.2009.01.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated protein genes (cas genes) have been suggested to act as an immune system in archaea and bacteria mimicking the eukaryotic RNA interference (RNAi) system. We have investigated the properties of the protein SSO2001 from Sulfolobus solfataricus (Sso) P2, which is part of the cas gene cluster. This study shows that SSO2001 is an endonuclease specifically digesting double-stranded oligonucleotides and preferably cleaving at G: C pairs. Point mutations identify both highly conserved aspartate and glutamate residues as being crucial for the nuclease activity. The catalytic activity shows an optimum at neutral pH and pH 3. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：771 / 776

页数：6

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