Cellular assessment of the extract of bambangan (Mangifera pajang) as a potential cytoprotective agent for the human hepatocellular HepG2 cell line

被引：10

作者：

Abu Bakar, Mohd Fadzelly ^{[1
]}

Mohamed, Maryati ^{[2
]}

Rahmat, Asmah ^{[3
]}

Burr, Steven A. ^{[4
]}

Fry, Jeffrey R. ^{[4
]}

机构：

[1] Univ Malaysia Sabah, Inst Trop Biol & Conservat, Lab Phytochem & Phytomed, Kota Kinabalu 88400, Sabah, Malaysia

[2] Univ Tun Hussein Onn Malaysia, Fac Civil & Environm Engn, Batu Pahat 86400, Johor, Malaysia

[3] Univ Putra Malaysia, Fac Med & Hlth Sci, Dept Nutr & Dietet, Serdang 43400, Selangor, Malaysia

[4] Univ Nottingham, Sch Med, Sch Biomed Sci, Queens Med Ctr, Nottingham NG7 2UH, England

来源：

FOOD CHEMISTRY | 2013年 / 136卷 / 01期

关键词：

Bambangan (Mangifera pajang); Cytoprotection; Western blotting; Cytoprotective proteins; ANTIOXIDANT DEFENSE; QUINONE REDUCTASE; OXIDATIVE DAMAGE; GENE-EXPRESSION; IN-VITRO; INDUCTION; PROTECT; CANCER; CHEMOPREVENTION; PREVENTION;

D O I：

10.1016/j.foodchem.2012.07.099

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study was conducted to investigate the potential of bambangan (Mangifera pajang) fruit extracts in the protection against oxidative damage caused by tert-butyl hydroperoxide in the human hepatocellular HepG2 cell line. Proteins which might be involved in the cytoprotective mechanism were investigated using western blotting technique. Quercetin was used as a positive control. The results showed that only the kernel extract of M. pajang and quercetin displayed cytoprotective activity in HepG2 cells, with EC50 values of 1.2 and 5.3 mu g/ml, respectively. Expression of quinone reductase, glutathione reductase and methionine sulfoxide reductase A proteins were significantly up-regulated by quercetin, suggesting their involvement in the cytoprotective activity of quercetin. However, expressions of only glutathione reductase and methionine sulfoxide reductase A proteins were significantly up-regulated by the kernel extract, again suggesting their involvement in the cytoprotective activity of bambangan kernel extract. Future study is needed to investigate the involvement of other cytoprotective proteins in the cytoprotection mechanism. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：18 / 25

页数：8

共 27 条

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