The Arabidopsis Thylakoid ADP/ATP Carrier TAAC Has an Additional Role in Supplying Plastidic Phosphoadenosine 5′-Phosphosulfate to the Cytosol

被引：69

作者：

Gigolashvili, Tamara ^{[1
]}

Geier, Melanie ^{[2
]}

Ashykhmina, Natallia ^{[1
]}

Frerigmann, Henning ^{[1
]}

Wulfert, Sabine ^{[1
]}

Krueger, Stephan ^{[1
]}

Mugford, Sarah G. ^{[3
]}

Kopriva, Stanislav ^{[3
]}

Haferkamp, Ilka ^{[2
]}

Fluegge, Ulf-Ingo ^{[1
]}

机构：

[1] Univ Cologne, Cologne Bioctr, Inst Bot, D-50674 Cologne, Germany

[2] Tech Univ Kaiserslautern, D-67663 Kaiserslautern, Germany

[3] John Innes Ctr, Dept Metab Biol, Norwich NR4 7UH, Norfolk, England

来源：

PLANT CELL | 2012年 / 24卷 / 10期

基金：

英国生物技术与生命科学研究理事会;

关键词：

ADENINE-NUCLEOTIDE TRANSPORTER; GLUCOSINOLATE BIOSYNTHESIS; PROTEIN IMPORT; TYROSYLPROTEIN SULFOTRANSFERASE; TRANSCRIPTION FACTORS; TRANSIENT EXPRESSION; MOLECULAR-CLONING; ATP/ADP CARRIER; IDENTIFICATION; PATHWAY;

D O I：

10.1105/tpc.112.101964

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

3'-Phosphoadenosine 5'-phosphosulfate (PAPS) is the high-energy sulfate donor for sulfation reactions. Plants produce some PAPS in the cytosol, but it is predominantly produced in plastids. Accordingly, PAPS has to be provided by plastids to serve as a substrate for sulfotransferase reactions in the cytosol and the Golgi apparatus. We present several lines of evidence that the recently described Arabidopsis thaliana thylakoid ADP/ATP carrier TAAC transports PAPS across the plastid envelope and thus fulfills an additional function of high physiological relevance. Transport studies using the recombinant protein revealed that it favors PAPS, 3'-phosphoadenosine 5'-phosphate, and ATP as substrates; thus, we named it PAPST1. The protein could be detected both in the plastid envelope membrane and in thylakoids, and it is present in plastids of autotrophic and heterotrophic tissues. TAAC/PAPST1 belongs to the mitochondrial carrier family in contrast with the known animal PAPS transporters, which are members of the nucleotide- sugar transporter family. The expression of the PAPST1 gene is regulated by the same MYB transcription factors also regulating the biosynthesis of sulfated secondary metabolites, glucosinolates. Molecular and physiological analyses of papst1 mutant plants indicate that PAPST1 is involved in several aspects of sulfur metabolism, including the biosynthesis of thiols, glucosinolates, and phytosulfokines.

引用

页码：4187 / 4204

页数：18

共 85 条

[11] Targeting of thylakoid proteins by the ΔpH-driven twin-arginine translocation pathway requires a specific signal in the hydrophobic domain in conjunction with the twin-arginine motif [J].