Anthocyanins attenuate body weight gain via modulating neuropeptide Y and GABAB1 receptor in rats hypothalamus

被引:66
作者
Badshah, Haroon [1 ]
Ullah, Ikram [1 ]
Kim, So Eun [1 ]
Kim, Tae-hyun [1 ]
Lee, Hae Young [1 ]
Kim, Myeong Ok [1 ]
机构
[1] Gyeongsang Natl Univ, Coll Nat Sci RINS, Dept Biol, Jinju 660701, South Korea
基金
新加坡国家研究基金会;
关键词
Obesity; Anthocyanins; Neuropeptide Y; GABA(B1) receptor; p-CREB; PKA; PARAVENTRICULAR NUCLEUS; ENERGY-BALANCE; ARCUATE NUCLEUS; OBESITY; MECHANISMS; APPETITE; INHIBIT; NPY; HYPERGLYCEMIA; ANTIOBESITY;
D O I
10.1016/j.npep.2013.06.001
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Anthocyanins in a variety of plant species have been identified and are known for its hypolipidemic and anti-obesity effects. The effect of anthocyanins extracted from black soybean on body weight and daily food intake in adult rats raised on normal diet were studied. Male Sprague-Dawley rats were daily intra-gastric administered water or anthocyanins 6 mg/kg and 24 mg/kg for 40 days. During this period daily food intake and body weight were measured prior to anthocyanins treatment. These findings showed that anthocyanins treatment resulted in significantly lowered body weight and food intake compared with water treated rats. In addition, anthocyanins dose dependently reduced the adipose tissue size compared with control group. Western blot analysis showed that high dose of anthocyanins treatment significantly reduced the expression of neuropeptide Y (NPY) and increased gamma-amino butyric acid receptor (GABA(B1)R) in hypothalamus. Furthermore, these events were followed by a decreased in expression of GABA(B1)R downstream signaling molecules protein kinase A-alpha (PKA) and phosphorylated cAMP-response element binding protein (p-CREB) in hypothalamus. These data support the concept that anthocyanins even in normal circumstances have the capability to reduce body weight and food intake through its modulatory effect on NPY and GABA(B1)R in hypothalamus. These results suggest that anthocyanins from black soybean seed coat might have a novel role in preventing obesity in rats on normal diet. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:347 / 353
页数:7
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