Consecutive Signal Amplification for DNA Detection Based on De Novo Fluorophore Synthesis and Host-Guest Chemistry

被引：37

作者：

Chen, Xiao-Hua ^{[1
]}

Roloff, Alexander ^{[1
]}

Seitz, Oliver ^{[1
]}

机构：

[1] Humboldt Univ, Dept Chem, D-12489 Berlin, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 18期

关键词：

DNA detection; DNA-templated catalysis; fluorescence; host-guest interactions; TEMPLATED ORGANIC-SYNTHESIS; TRIGGERED CATALYTIC DRUG; NUCLEIC-ACID DETECTION; FLUOROGENIC REACTION; CHEMICAL LIGATION; PROBE ACTIVATION; METAL CATALYSIS; RNA; VERSATILE; RELEASE;

D O I：

10.1002/anie.201108845

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two is more than one: A method has been developed in which a DNA template triggers a Wittig reaction that leads to transfer of a benzylidene group from a DNA-linked phosphonium salt to a DNA-linked benzaldehyde. A synthetic receptor such as α-cyclodextrin then encapsulates the newly formed stilbene and triggers further signal increases. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：4479 / 4483

页数：5

共 58 条

[21] 7-Azidomethoxy-Coumarins as Profluorophores for Templated Nucleic Acid Detection [J].