Target-catalyzed transfer reactions for the amplified detection of RNA

被引：59

作者：

Grossmann, Tom N. ^{[1
]}

Roeglin, Lars ^{[1
]}

Seitz, Oliver ^{[1
]}

机构：

[1] Humboldt Univ, Inst Chem, D-12489 Berlin, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 37期

关键词：

analytical methods; nucleic acids; RNA detection; signal amplification; template catalysis;

D O I：

10.1002/anie.200801355

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Strong signals: The RNA-catalyzed transfer of a biotin reporter was used to preamplify a signal that was detected in an enzyme-based readout, thereby increasing its sensitivity. The advantages of combining the covalent attachment of biotin and preamplification were demonstrated by the detection of 500 attomol HIV RNA. The flexibility requirements of DNA- and RNA-catalyzed transfer reactions were tested. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：7119 / 7122

页数：4

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