Improved nucleic acid triggered probe activation through the use of a 5-thiomethyluracil peptide nucleic acid building block

被引：41

作者：

Cai, JF ^{[1
]}

Li, XX ^{[1
]}

Taylor, JS ^{[1
]}

机构：

[1] Washington Univ, Dept Chem, St Louis, MO 63130 USA

来源：

ORGANIC LETTERS | 2005年 / 7卷 / 05期

关键词：

D O I：

10.1021/ol0478382

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

To improve the efficiency of a nucleic acid triggered probe activation (NATPA) system a 5-thiomethyluracil peptide nucleic acid (PNA) building block has been synthesized. Attachment of imidazole and a coumarin ester to uracils at the ends of two PNAs resulted in a 550 000-fold acceleration of DNA-triggered coumarin release relative to imidazole and a 6-fold increase in k(cat) relative to a system which had these groups attached to the amino and carboxy ends of PNAs.

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页码：751 / 754

页数：4

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