Reducing product inhibition in DNA-template-controlled ligation reactions

被引：90

作者：

Dose, Christian ^{[1
]}

Ficht, Simon ^{[1
]}

Seitz, Oliver ^{[1
]}

机构：

[1] Humboldt Univ, Inst Chem, D-12489 Berlin, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 32期

关键词：

FRET (fluorescence resonance energy transfer); native chemical ligation; sequence analysis; single-nucleotide polymorphism; template synthesis;

D O I：

10.1002/anie.200600464

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Loosening the zipper: A ligation-rearrangement reaction sequence is the key to signal amplification in chemical DNA-templated ligation reactions. The rearrangement increases the flexibility of the ligation intermediate, thereby reducing the affinity of the rearranged product for the DNA template. A fluorescence-resonant-energy-transfer technique is used for convenient and product-specific real-time detection of product signals. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：5369 / 5373

页数：5

相关论文

共 41 条

[1] Destabilizing universal linkers for signal amplification in self-ligating probes for RNA [J].

Abe, H ;

Kool, ET .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (43) :13980-13986

[2] Chemical amplification (CHAMP) by a continuous, self-replicating oligonucleotide-based system [J].

Albagli, D ;

Van Atta, R ;

Cheng, P ;

Huan, BF ;

Wood, ML .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (29) :6954-6955

[3] Templated metal catalysis for single nucleotide specific DNA sequence detection [J].

Boll, I ;

Krämer, R ;

Brunner, J ;

Mokhir, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (21) :7849-7856

[4] DNA-templated metal catalysis [J].

Brunner, J ;

Mokhir, A ;

Kraemer, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (41) :12410-12411

[5] Improved nucleic acid triggered probe activation through the use of a 5-thiomethyluracil peptide nucleic acid building block [J].

Cai, JF ;

Li, XX ;

Taylor, JS .

ORGANIC LETTERS, 2005, 7 (05) :751-754

[6] Nucleic acid-triggered fluorescent probe activation by the staudinger reaction [J].

Cai, JF ;

Li, XX ;

Yue, X ;

Taylor, JS .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (50) :16324-16325

[7] SYNTHESIS OF PROTEINS BY NATIVE CHEMICAL LIGATION [J].

DAWSON, PE ;

MUIR, TW ;

CLARKLEWIS, I ;

KENT, SBH .

SCIENCE, 1994, 266 (5186) :776-779

[8]

Diederich F., 1999, TEMPLATED ORGANIC SY

[9] New isocysteine building blocks and chemoselective peptide ligation [J].

Dose, C ;

Seitz, O .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2004, 2 (01) :59-65

[10] DNA nanotechnology: Chemical copying of connectivity [J].

Eckardt, LH ;

Naumann, K ;

Pankau, WM ;

Rein, M ;

Schweitzer, M ;

Windhab, N ;

von Kiedrowski, G .

NATURE, 2002, 420 (6913) :286-286

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