Attachment, proliferation and differentiation of BMSCs on gas-jet/electrospun nHAP/PHB fibrous scaffolds

被引：41

作者：

Guan, Donghua ^{[1
]}

Chen, Zhiqing ^{[1
]}

Huang, Chunpeng ^{[1
]}

Lin, Yinghe ^{[1
,2
]}

机构：

[1] Sichuan Univ, State Key Lab Oral Dis, Chengdu 610041, Peoples R China

[2] W China Stomatol Hosp, Chengdu 610041, Peoples R China

来源：

APPLIED SURFACE SCIENCE | 2008年 / 255卷 / 02期

关键词：

Poly(3-hydroxybutyrate); Nano-hydroxyapatite; Electrospinning; Tissue engineering;

D O I：

10.1016/j.apsusc.2008.06.093

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 [物理化学]; 081704 [应用化学];

摘要：

In this study, poly(3-hydroxybutyrate) (PHB)-based scaffolds containing nanosized hydroxyapatite (nHAP) were manufactured by gas-jet/electrospinning. The morphologies of the scaffolds were characterized. The effect of the scaffolds on attachment, proliferation and differentiation of the bone marrow stroma cells (BMSCs) were accessed by using scanning electron microscopy (SEM), methylthiazol tetrazolium (MTT) assay and alkaline phosphatase (ALP) activity. The results show that the gas-jet/electrospun scaffolds possess an extracellular matrix-like topography. In vitro studies describe that the scaffolds have positive effects on attachment, proliferation and differentiation of BMSCs in vitro. It can be concluded that the scaffolds combing the unique structural features generated by gas-jet/electrospinning with functional factors, have the potential to be used in bone tissue engineering. (C) 2008 Elsevier B. V. All rights reserved.

引用

页码：324 / 327

页数：4

共 17 条

[1]

Controlling surface morphology of electrospun polystyrene fibers: Effect of humidity and molecular weight in the electrospinning process [J].