Rapid identification of hyperthermophilic methanococci isolated from deep-sea hydrothermal vents

被引：23

作者：

Jeanthon, C

L'Haridon, S

Pradel, N

Prieur, D

机构：

[1] CNRS, Biol Stn, F-29680 Roscoff, France

[2] Univ Paris 06, F-29680 Roscoff, France

[3] Univ Bretagne Occidentale, UFR Sci & Tech, F-29285 Brest, France

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY | 1999年 / 49卷

关键词：

Methanococcus; RFLP; 16S rDNA; Archaea; hydrothermal vents;

D O I：

10.1099/00207713-49-2-591

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

16S rDNAs amplified by PCR from 22 hyperthermophilic methanococci isolated from deep-sea hydrothermal vents were compared with those of the six type strains of the genus Methanococcus by RFLP analysis. Restriction fragments obtained with HaeIII enabled four of the type species to be distinguished. Restrictions with Hhal, BstUI and Mspl were necessary to differentiate Methanococcus jannaschii and Methanococcus fervens. The results indicate that the 16S rDNA PCR-RFLP method provides a rapid and reliable tool for the identification of newly isolated hyperthermophilic Methanococcus spp.

引用

页码：591 / 594

页数：4

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