Cocaine Detection in Blood Serum Using Aptamer Biosensor on Gold Nanoparticles and Progressive Dilution

被引：18

作者：

Ge Jing

Liu Zhaofang

Zhao Xin Sheng ^{[1
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, Dept Biol Chem,Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China

来源：

CHINESE JOURNAL OF CHEMISTRY | 2012年 / 30卷 / 09期

关键词：

aptamer; biosensors; cocaine; serum; progressive dilution; AU NANOPARTICLES; DNA APTAMER; SENSOR; PROTEIN; CANCER; MOLECULES; ADENOSINE; SELECTION; THROMBIN; DYNAMICS;

D O I：

10.1002/cjoc.201200256

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

To fight against the illegal usage of cocaine it is necessary to develop various analytical methodologies. We report here an aptamer-based biosensor for the determination of cocaine using gold nanoparticles as the fluorescence quencher. By employing the progressive dilution (PD) strategy, simultaneous qualitative and quantitative analysis of cocaine in blood serum was achieved without pretreatment of the sample. The method described in this paper offers significant improvement in the detection accuracy and can be used to quantify cocaine levels in complex biological samples such as serum with a simple procedure.

引用

页码：2023 / 2028

页数：6

共 31 条

[1]

SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].

BOCK, LC ;

GRIFFIN, LC ;

LATHAM, JA ;

VERMAAS, EH ;

TOOLE, JJ .

NATURE, 1992, 355 (6360) :564-566

[2]

Aptamers come of age - at last [J].

Bunka, David H. J. ;

Stockley, Peter G. .

NATURE REVIEWS MICROBIOLOGY, 2006, 4 (08) :588-596

[3]

Enzymatic Manipulation of DNA-Modified Gold Nanoparticles for Screening G-Quadruplex Ligands and Evaluating Selectivities [J].

Chen, Cuie ;

Zhao, Chuanqi ;

Yang, Xinjian ;

Ren, Jinsong ;

Qu, Xiaogang .

ADVANCED MATERIALS, 2010, 22 (03) :389-+

[4]

Base-by-Base Dynamics in DNA Hybridization Probed by Fluorescence Correlation Spectroscopy [J].

Chen, Xudong ;

Zhou, Yan ;

Qu, Peng ;

Zhao, Xin Sheng .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (50) :16947-16952

[5]

A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles [J].

Demers, LM ;

Mirkin, CA ;

Mucic, RC ;

Reynolds, RA ;

Letsinger, RL ;

Elghanian, R ;

Viswanadham, G .

ANALYTICAL CHEMISTRY, 2000, 72 (22) :5535-5541

[6]

INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

[7]

Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution [J].

Ge, Jing ;

Liu, Zhaofang ;

Zhi, Zeyong ;

Zhao, Xin Sheng .

ANALYTICAL BIOCHEMISTRY, 2011, 413 (01) :75-77

[8]

Fluorescence Aptameric Sensor for Strand Displacement Amplification Detection of Cocaine [J].

He, Jing-Lin ;

Wu, Zai-Sheng ;

Zhou, Hui ;

Wang, Hong-Qi ;

Jiang, Jian-Hui ;

Shen, Guo-Li ;

Yu, Ru-Qin .

ANALYTICAL CHEMISTRY, 2010, 82 (04) :1358-1364

[9]

Nanoparticle-Based, Fluorous-Tag-Driven DNA Detection [J].

Hong, Min ;

Zhou, Xin ;

Lu, Zhiqiang ;

Zhu, Jin .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (50) :9503-9506

[10]

A DNA APTAMER THAT BINDS ADENOSINE AND ATP [J].

HUIZENGA, DE ;

SZOSTAK, JW .

BIOCHEMISTRY, 1995, 34 (02) :656-665

← 1 2 3 4 →