Spindle imaging: a new marker for optimal timing of ICSI?

被引：87

作者：

Cohen, Y ^{[1
]}

Malcov, M ^{[1
]}

Schwartz, T ^{[1
]}

Mey-Raz, N ^{[1
]}

Carmon, A ^{[1
]}

Cohen, T ^{[1
]}

Lessing, JB ^{[1
]}

Amit, A ^{[1
]}

Azem, F ^{[1
]}

机构：

[1] Tel Aviv Univ, Tel Aviv Sourasky Med Ctr, In Vitro Fertilizat Unit, Lis Matern Hosp,Sackler Fac Med, IL-64239 Tel Aviv, Israel

来源：

HUMAN REPRODUCTION | 2004年 / 19卷 / 03期

关键词：

HCG; ICSI; oocytes; Polscope; spindle;

D O I：

10.1093/humrep/deh113

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Background: The LC Polscope facilitates visualization of the meiotic spindle in human oocyte. This study aimed to investigate meiotic spindle assembly in correlation to time elapsed after HCG administration, and to determine whether spindle imaging may serve to indicate the likelihood of fertilization and embryo cleavage. Methods: Metaphase II (MII) oocytes from 103 couples who were being treated for male infertility were imaged with the Polscope prior to sperm injection. Spindle imaging was correlated to time elapsed from HCG administration, fertilization rate and embryo cleavage. The main outcome measures were spindle visualization, fertilization and embryo cleavage on day 3. Results: A total of 770 MII oocytes were imaged. A spindle was imaged in a significantly higher number of oocytes from greater than or equal to38 h after HCG administration compared with those in the <38 h group (78.1-81.5% versus 61.6%; P < 0.001). The fertilization rate in oocytes with a visible spindle was statistically higher compared with oocytes in which spindle could not be detected (70.4% versus 62.2%; P = 0.035). We found no relationship between spindle imaging and embryo cleavage on day 3. Conclusions: Spindle imaging, in addition to first polar body appearance, is an accurate indicator for oocyte maturity. We suggest that spindle imaging be performed prior to sperm injection.

引用

页码：649 / 654

页数：6

共 21 条

[1] GENE ACTIVITY AND CLEAVAGE ARREST IN HUMAN PREEMBRYOS [J].