An in vitro biological and anti-bacterial study on a sol-gel derived silver-incorporated bioglass system

被引:210
作者
Balamurugan, A. [1 ]
Balossier, G. [2 ]
Laurent-Maquin, D. [3 ]
Pina, S. [1 ]
Rebelo, A. H. S. [1 ]
Faure, J. [2 ]
Ferreira, J. M. F. [1 ]
机构
[1] Univ Aveiro, Dept Ceram & Glass Engn, CICECO, P-3810193 Aveiro, Portugal
[2] Univ Reims, INSERM, Lab Microscopie Elect Analyt, ERM 0203, F-51685 Reims 2, France
[3] Univ Reims, INSERM, ERM 0203, F-51096 Reims 2, France
关键词
sol-gel; bacteriostatic; silver; biomaterial;
D O I
10.1016/j.dental.2008.02.015
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Objectives. The purpose of this study was to evaluate the in vitro antibacterial and biological activity of silver-incorporated bioactive glass system SiO2-CaO-P2O5-Ag2O (AgBG). The bacteriostatic and bactericidal proper-ties of this new quaternary glass system along with the ternary sol-gel glass system SiO2-CaO-P2O5 (BG) have been studied using Escherichia coli as a test micro-organism. The AGBG system thus appears to be a promising material for dental applications, since similar effects might be produced on a film of bacteria and mucous that grows on the teeth. Methods. The SiO2-CaO-P2O5-Ag2O and SiO2-CaO-P2O5 glass systems were synthesized by the sol-gel technique and characterized for their physicho-chemical properties. The antibacterial activity and biological properties were evaluated by determining the minimum inhibitory concentrations (MICs). Release of Ag+ into the culture medium was measured by inductively coupled plasma (ICP) analysis. Results. The in vitro antibacterial action of the SiO2-CaO-P2O5-Ag2O was compared with that of its ternary counterpart glass system. The concentrations of Ag-bioglass, in the range of 0.02-0.20 mg of Ag-bioglass per millilitre of culture medium, were found to inhibit the growth of these bacteria. The Ag-bioglass not only acts bacteriostatically but it also elicited a rapid bactericidal action. A complete bactericidal effect was elicited in the early stages of the incubation at Ag-bioglass concentration of 20mg/ml and the ternary glass system had no effect on bacterial growth or viability. The antibacterial action of Ag-bioglass was exclusively attributed to the leaching of Ag+ ions from the glass matrix. Significance. One of the major advantages of incorporating silver ions into a gel glass system is that the porous glass matrix can allow for controlled sustained delivery of the antibacterial agent to dental material, used even under anaerobic conditions such as deep in the periodontal pocket. This glass system also provides long-term action required for systems which are constantly at risk of microbial contamination. (C) 2008 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:1343 / 1351
页数:9
相关论文
共 43 条
  • [1] Antibacterial properties of a silver chloride-coated nylon wound dressing
    Adams, AP
    Santschi, EM
    Mellencamp, MA
    [J]. VETERINARY SURGERY, 1999, 28 (04) : 219 - 225
  • [2] *AM SOC MICR, 1981, MAN METH GEN BACT
  • [3] [Anonymous], 92771995E ISO
  • [4] [Anonymous], 1989, HDB INDUCTIVELY COUP
  • [5] Rubidium 'whiskers' in a vapour cell
    Balabas, M. V.
    Sushkov, A. O.
    Budker, D.
    [J]. NATURE PHYSICS, 2007, 3 (01) : 2 - 2
  • [6] Development and characterisation of silver-doped bioactive glasscoated sutures for tissue engineering and wound healing applications
    Blaker, JJ
    Nazhat, SN
    Boccaccini, AR
    [J]. BIOMATERIALS, 2004, 25 (7-8) : 1319 - 1329
  • [7] Silver coated materials for external fixation devices:: in vitro biocompatibility and genotoxicity
    Bosetti, M
    Massè, A
    Tobin, E
    Cannas, M
    [J]. BIOMATERIALS, 2002, 23 (03) : 887 - 892
  • [8] Bioactive materials
    Cao, WP
    Hench, LL
    [J]. CERAMICS INTERNATIONAL, 1996, 22 (06) : 493 - 507
  • [9] CARLISLE EM, 1996, SILICON BIOCH, P123
  • [10] THERAPEUTIC EFFECTS OF SILVER NYLON DRESSINGS WITH WEAK DIRECT-CURRENT ON PSEUDOMONAS-AERUGINOSA-INFECTED BURN WOUNDS
    CHU, CS
    MCMANUS, AT
    PRUITT, BA
    MASON, AD
    [J]. JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE, 1988, 28 (10) : 1488 - 1492