Development and application of a one-step low cost procedure to concentrate viruses from seawater samples

被引：126

作者：

Calgua, B. ^{[1
]}

Mengewein, A. ^{[2
]}

Grunert, A. ^{[2
]}

Bofill-Mas, S. ^{[1
]}

Clemente-Casares, P. ^{[1
]}

Hundesa, A. ^{[1
]}

Wyn-Jones, A. P. ^{[3
]}

Lopez-Pila, J. M. ^{[2
]}

Girones, R. ^{[1
]}

机构：

[1] Univ Barcelona, Fac Biol, Dept Microbiol, E-08028 Barcelona, Spain

[2] German Environm Agcy Umweltbundesamt, D-06844 Berlin, Germany

[3] Aberystwyth Univ, IGES, Aberystwyth SY23 3DB, Dyfed, Wales

来源：

JOURNAL OF VIROLOGICAL METHODS | 2008年 / 153卷 / 02期

关键词：

Adenovirus; Seawater; Flocculation; Concentration method; QPCR;

D O I：

10.1016/j.jviromet.2008.08.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel and simple procedure for concentrating adenoviruses from seawater samples is described. The technique entails the adsorption of viruses to pre-flocculated skimmed milk proteins, allowing the flocs to sediment by gravity, and dissolving the separated sediment in phosphate buffer. Concentrated virus may be detected by PCR techniques following nucleic acid extraction. The method requires no specialized equipment other than that usually available in routine public health laboratories, and due to its straightforwardness it allows the processing of a larger number of water samples simultaneously. The usefulness of the method was demonstrated in concentration of virus in multiple seawater samples during a survey of adenoviruses in coastal waters. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：79 / 83

页数：5

共 26 条

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