Fast chromatin immunoprecipitation assay

被引：184

作者：

Nelson, JD

Denisenko, O

Sova, P

Bomsztyk, K

机构：

[1] Univ Washington, UW Med Lake Union, Seattle, WA 98109 USA

[2] Univ Washington, Mol & Cellular Biol Program, Seattle, WA 98109 USA

来源：

NUCLEIC ACIDS RESEARCH | 2006年 / 34卷 / 01期

关键词：

D O I：

10.1093/nar/gnj004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatin immunoprecipitation (ChIP) is a widely used method to explore in vivo interactions between proteins and DNA. The ChIP assay takes several days to complete, involves several tube transfers and uses either phenol-chlorophorm or spin columns to purify DNA. The traditional ChIP method becomes a challenge when handling multiple samples. We have developed an efficient and rapid Chelex resin-based ChIP procedure that dramatically reduces time of the assay and uses only a single tube to isolate PCR-ready DNA. This method greatly facilitates the probing of chromatin changes over many time points with several antibodies in one experiment.

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页数：7

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