An RNA sensor for intracellular Mg2+

被引:257
作者
Cromie, MJ [1 ]
Shi, YX [1 ]
Latifi, T [1 ]
Groisman, EA [1 ]
机构
[1] Washington Univ, Sch Med, Howard Hughes Med Inst, Dept Mol Microbiol, St Louis, MO 63110 USA
关键词
D O I
10.1016/j.cell.2006.01.043
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Most RNA molecules require Mg2+ for their structure and enzymatic properties. Here we report the first example of an RNA serving as sensor for cytoplasmic Mg2+. We establish that expression of the Mg2+ transporter MgtA of Salmoneila enterica serovar Typhimurium is controlled by its 51 untranslated region (5'UTR). We show that the 5'UTR of the mgtA gene can adopt different stem-loop structures depending on the Mg2+ levels, which determine whether transcription reads through into the mgtA coding region or stops within the 5'UTR. We could recapitulate the Mg2+-regulated transcription using a defined in vitro transcription system with RNA polymerase as the only protein component. The initiation of mgtA transcription responds to extracytoplasmic Mg2+ and its elongation into the coding region to cytoplasmic Mg2+, providing a singular example in which the same ligand is sensed in different cellular compartments to regulate disparate steps in gene transcription.
引用
收藏
页码:71 / 84
页数:14
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