Trends and challenges in enzyme technology

被引：39

作者：

Bornscheuer, UT ^{[1
]}

机构：

[1] Ernst Moritz Arndt Univ Greifswald, Dept Tech Chem & Biotechnol, Inst Chem & Biochem, D-17487 Greifswald, Germany

来源：

BIOTECHNOLOGY FOR THE FUTURE | 2005年 / 100卷

关键词：

biocatalysis; directed evolution; immobilization; biodiversity; metagenome; high-throughput screening; dynamic kinetic resolution;

D O I：

10.1007/sb136413

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Several major developments took place in the field of biocatalysis over the past few years. These include the invention of directed evolution as an extremely useful method for biocatalyst improvement on the molecular level in combination with high-throughput screening systems, methods for accessing "nonculturable" biodiversity using metagenome approaches and progress in sequence-based biocatalyst discovery. In addition, new carriers and tools for immobilization of enzymes have been developed. For the synthesis of optically active compounds impressive examples using new enzymes and major progress in dynamic kinetic resolutions of racemates took place. These achievements are summarized in this review.

引用

页码：181 / 203

页数：23

共 102 条

[21]

2-B

[22] Directed evolution of enzymes for biocatalytic applications [J].