Directed evolution of an amine oxidase possessing both broad substrate specificity and high enantioselectivity

被引:139
作者
Carr, R
Alexeeva, M
Enright, A
Eve, TSC
Dawson, MJ
Turner, NJ
机构
[1] Univ Edinburgh, Sch Chem, Edinburgh EH9 3JJ, Midlothian, Scotland
[2] GlaxoSmithKline R&D, Med Res Ctr, Stevenage SG1 2NY, Herts, England
关键词
amines; deracemization; directed evolution; enzymes;
D O I
10.1002/anie.200352100
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Evolving enzymes: Directed evolution of the amine oxidase from Aspergillus niger, by using (S)-α-methylbenzylamine as the target substrate, resulted in a variant enzyme (Asn336Ser) possessing both broad substrate specificity and high S enantioselectivity towards a wide range of chiral amines (see scheme).
引用
收藏
页码:4807 / 4810
页数:4
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