Directed evolution of an amine oxidase possessing both broad substrate specificity and high enantioselectivity

被引：139

作者：

Carr, R

Alexeeva, M

Enright, A

Eve, TSC

Dawson, MJ

Turner, NJ

机构：

[1] Univ Edinburgh, Sch Chem, Edinburgh EH9 3JJ, Midlothian, Scotland

[2] GlaxoSmithKline R&D, Med Res Ctr, Stevenage SG1 2NY, Herts, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2003年 / 42卷 / 39期

关键词：

amines; deracemization; directed evolution; enzymes;

D O I：

10.1002/anie.200352100

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Evolving enzymes: Directed evolution of the amine oxidase from Aspergillus niger, by using (S)-α-methylbenzylamine as the target substrate, resulted in a variant enzyme (Asn336Ser) possessing both broad substrate specificity and high S enantioselectivity towards a wide range of chiral amines (see scheme).

引用

页码：4807 / 4810

页数：4

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