Induced systemic resistance in cucumber and Arabidopsis thaliana by the combination of Trichoderma harzianum Tr6 and Pseudomonas sp Ps14

Trichoderma species and fluorescent Pseudomonas spp. have been reported to induce systemic resistance in plants. In this study the effectiveness of a combination of these biological control agents on the efficacy of induced resistance was investigated in cucumber and the model plant Arabidopsis thaliana. Trichoderma harzianum Tr6, and Pseudomonas sp. Ps14, both isolated from the rhizosphere of cucumber, were tested as a single application and in combination for their abilities to elicit induced resistance in cucumber against Fusarium oxysporum f. sp. radicis cucumerinum and in A. thaliana against Botrytis cinerea. The combination of Tr6 and Ps14 induced a significantly higher level of resistance in cucumber, which was associated with the primed expression of a set of defense-related genes upon challenge with Fusarium. In Arabidopsis both Ps14 and Tr6 triggered ISR against B. cinerea but their combination did not show enhanced effects. In the induced systemic resistance-defective Arabidopsis mutant myb72, none of the treatments protected against B. cinerea, whereas in the SA-impaired mutant sid2 all treatments were effective. Taken together, these results indicate that in Arabidopsis Ps14 and Tr6 activate the same signaling pathway and thus have no enhanced effect in combination. The enhanced protection in cucumber by the combination is most likely due to activation of different signaling pathways by the two biocontrol agents. (C) 2013 Elsevier Inc. All rights reserved.