Liquid injection gas chromatography and the ALDH assay overestimate free acetaldehyde in complex fermentation media

被引：14

作者：

Hobley, TJ ^{[1
]}

Pamment, NB ^{[1
]}

机构：

[1] UNIV MELBOURNE,DEPT CHEM ENGN,PARKVILLE,VIC 3052,AUSTRALIA

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 01期

关键词：

D O I：

10.1007/BF02764449

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Almost 75% of the free acetaldehyde added to an uninoculated culture medium was lost upon overnight incubation due to reaction with yeast extract and tryptone. In the presence of these components, liquid injection gas chromatography and the ALDH assay substantially overestimated acetaldehyde concentrations, highlighting the importance of head space gas chromatography in obtaining reliable estimates of the free acetaldehyde concentration.

引用

页码：39 / 42

页数：4

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