Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics

被引：180

作者：

Chokkalingam, Venkatachalam ^{[1
]}

Tel, Jurjen ^{[2
,3
]}

Wimmers, Florian ^{[2
,3
]}

Liu, Xin ^{[4
]}

Semenov, Sergey ^{[1
]}

Thiele, Julian ^{[1
]}

Figdor, Carl G. ^{[2
,3
]}

Huck, Wilhelm T. S. ^{[1
]}

机构：

[1] Radboud Univ Nijmegen, Inst Mol & Mat, NL-6525 AJ Nijmegen, Netherlands

[2] Radboud Univ Nijmegen, Med Ctr, Dept Tumor Immunol, NL-6500 HB Nijmegen, Netherlands

[3] Nijmegen Ctr Mol Life Sci, NL-6500 HB Nijmegen, Netherlands

[4] Sphere Fluid, Cambridge, England

来源：

LAB ON A CHIP | 2013年 / 13卷 / 24期

基金：

欧洲研究理事会;

关键词：

KAPPA-B DYNAMICS; SINGLE CELLS; T-CELLS; MICRODROPLETS; SURFACTANTS; EXPRESSION; CHEMISTRY; PLATFORM; ASSAYS;

D O I：

10.1039/c3lc50945a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we present a platform to detect cytokine (IL-2, IFN-gamma, TNF-alpha) secretion of single, activated T-cells in droplets over time. We use a novel droplet-based microfluidic approach to encapsulate cells in monodisperse agarose droplets together with functionalized cytokine-capture beads for subsequent binding and detection of secreted cytokines from single cells. This method allows high-throughput detection of cellular heterogeneity and maps subsets within cell populations with specific functions.

引用

页码：4740 / 4744

页数：5

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