Phylogenetic analysis of the non-structural (NS) gene of influenza A viruses isolated from mallards in Northern Europe in 2005

被引:23
作者
Zohari, Siamak [1 ,2 ,3 ]
Gyarmati, Peter [1 ,2 ,3 ]
Ejdersund, Anneli [4 ]
Berglof, Ulla [4 ]
Thoren, Peter [4 ]
Ehrenberg, Maria [5 ]
Czifra, Gyorgy [4 ]
Belak, Sandor [1 ,2 ,3 ]
Waldenstrom, Jonas [6 ,7 ]
Olsen, Bjorn [6 ,7 ]
Berg, Mikael [1 ,2 ,3 ]
机构
[1] Natl Vet Inst SVA, Joint Res & Dev Unit Virol Immunobiol & Parasitol, SE-75189 Uppsala, Sweden
[2] SLU, Sect Parasitol & Virol, Dept Biomed Sci & Publ Hlth, SE-75189 Uppsala, Sweden
[3] Swedish Univ Agr Sci SLU, SE-75189 Uppsala, Sweden
[4] SVA, Unit Virol Immunobiol & Parasitol, SE-75189 Uppsala, Sweden
[5] Natl Vet Inst SVA, Unit Chem Environm & Feed Safety, SE-75189 Uppsala, Sweden
[6] Univ Uppsala Hosp, Infect Dis Sect, Dept Med Sci, SE-75185 Uppsala, Sweden
[7] Kalmar Univ, Sect Zoonot Ecol & Epidemiol, SE-32185 Kalmar, Sweden
基金
瑞典研究理事会;
关键词
NUCLEAR EXPORT; MESSENGER-RNAS; POLY(A)-BINDING PROTEIN; MIGRATORY WATERFOWL; H5N1; SEQUENCE; CIRCULATION; INHIBITION; INTERFERON; BINDING;
D O I
10.1186/1743-422X-5-147
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: Although the important role of the non-structural 1 (NS) gene of influenza A in virulence of the virus is well established, our knowledge about the extent of variation in the NS gene pool of influenza A viruses in their natural reservoirs in Europe is incomplete. In this study we determined the subtypes and prevalence of influenza A viruses present in mallards in Northern Europe and further analysed the NS gene of these isolates in order to obtain a more detailed knowledge about the genetic variation of NS gene of influenza A virus in their natural hosts. Results: A total number of 45 influenza A viruses of different subtypes were studied. Eleven haemagglutinin-and nine neuraminidase subtypes in twelve combinations were found among the isolated viruses. Each NS gene reported here consisted of 890 nucleotides; there were no deletions or insertions. Phylogenetic analysis clearly shows that two distinct gene pools, corresponding to both NS allele A and B, were present at the same time in the same geographic location in the mallard populations in Northern Europe. A comparison of nucleotide sequences of isolated viruses revealed a substantial number of silent mutations, which results in high degree of homology in amino acid sequences. The degree of variation within the alleles is very low. In our study allele A viruses displays a maximum of 5% amino acid divergence while allele B viruses display only 2% amino acid divergence. All the viruses isolated from mallards in Northern Europe possessed the typical avian ESEV amino acid sequence at the C-terminal end of the NSI protein. Conclusion: Our finding indicates the existence of a large reservoir of different influenza A viruses in mallards population in Northern Europe. Although our phylogenetic analysis clearly shows that two distinct gene pools, corresponding to both NS allele A and B, were present in the mallards populations in Northern Europe, allele B viruses appear to be less common in natural host species than allele A, comprising only about 13% of the isolates sequenced in this study.
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页数:13
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共 52 条
[31]   Large-scale sequence analysis of avian influenza isolates [J].
Obenauer, JC ;
Denson, J ;
Mehta, PK ;
Su, XP ;
Mukatira, S ;
Finkelstein, DB ;
Xu, XQ ;
Wang, JH ;
Ma, J ;
Fan, YP ;
Rakestraw, KM ;
Webster, RG ;
Hoffmann, E ;
Krauss, S ;
Zheng, J ;
Zhang, ZW ;
Naeve, CW .
SCIENCE, 2006, 311 (5767) :1576-1580
[32]   2 FUNCTIONAL DOMAINS OF THE INFLUENZA-VIRUS NS1 PROTEIN ARE REQUIRED FOR REGULATION OF NUCLEAR EXPORT OF MESSENGER-RNA [J].
QIAN, XY ;
ALONSOCAPLEN, F ;
KRUG, RM .
JOURNAL OF VIROLOGY, 1994, 68 (04) :2433-2441
[33]   THE INFLUENZA-VIRUS NS1 PROTEIN IS A POLY(A)-BINDING PROTEIN THAT INHIBITS NUCLEAR EXPORT OF MESSENGER-RNAS CONTAINING POLY(A) [J].
QIU, Y ;
KRUG, RM .
JOURNAL OF VIROLOGY, 1994, 68 (04) :2425-2432
[34]   Attenuation of equine influenza viruses through truncations of the NS1 protein [J].
Quinlivan, M ;
Zamarin, D ;
García-Sastre, A ;
Cullinane, A ;
Chambers, T ;
Palese, P .
JOURNAL OF VIROLOGY, 2005, 79 (13) :8431-8439
[35]   Influenza virus targets the mRNA export machinery and the nuclear pore complex [J].
Satterly, Neal ;
Tsai, Pei-Ling ;
van Deursen, Jan ;
Nussenzveig, Daniel R. ;
Wang, Yaming ;
Faria, Paula A. ;
Levay, Agata ;
Levy, David E. ;
Fontoura, Beatriz M. A. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (06) :1853-1858
[36]   Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: Amino acid sequence at the HA cleavage site as a marker of pathogenicity potential [J].
Senne, DA ;
Panigrahy, B ;
Kawaoka, Y ;
Pearson, JE ;
Suss, J ;
Lipkind, M ;
Kida, H ;
Webster, RG .
AVIAN DISEASES, 1996, 40 (02) :425-437
[37]   RETRACTED: The NS1 gene of H5N1 influenza viruses circumvents the host anti-viral cytokine responses (Retracted Article. See vol 149, pg 133, 2010) [J].
Seo, SH ;
Hoffmann, E ;
Webster, RG .
VIRUS RESEARCH, 2004, 103 (1-2) :107-113
[38]   Identification of sensitive and specific avian influenza polymerase chain reaction methods through blind ring trials organized in the European Union [J].
Slomka, M. J. ;
Coward, V. J. ;
Banks, J. ;
Londt, B. Z. ;
Brown, I. H. ;
Voermans, J. ;
Koch, G. ;
Handberg, K. J. ;
Jorgensen, P. H. ;
Cherbonnel-Pansart, M. ;
Jestin, V. ;
Cattoli, G. ;
Capua, I. ;
Ejdersund, A. ;
Thoren, P. ;
Czifra, G. .
AVIAN DISEASES, 2007, 51 (01) :227-234
[39]  
Spackman E, 2005, VIRUS RES, V114, P89, DOI 10.1016/j.virusres.2005.05.013
[40]   Development of real-time RT-PCR for the detection of avian influenza virus [J].
Spackman, E ;
Senne, DA ;
Bulaga, LL ;
Myers, TJ ;
Perdue, ML ;
Garber, LP ;
Lohman, K ;
Daum, LT ;
Suarez, DL .
AVIAN DISEASES, 2003, 47 :1079-1082