Medium optimization and potential hepatoprotective effect of mycelial polysaccharides from Pholiota dinghuensis Bi against carbon tetrachloride-induced acute liver injury in mice

被引：53

作者：

Gan, Dan ^{[1
,2
]}

Ma, Liping ^{[1
]}

Jiang, Changxing ^{[1
]}

Wang, Mingchun ^{[1
]}

Zeng, Xiaoxiong ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China

[2] Xuzhou Coll Ind Technol, Dept Chem Engn, Xuzhou 221140, Jiangsu, Peoples R China

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2012年 / 50卷 / 08期

关键词：

Pholiota dinghuensis Bi; Mycelial polysaccharide; Medium optimization; Hepatoprotective effect; RESPONSE-SURFACE METHODOLOGY; INDUCED HEPATOTOXICITY; EXTRACT; CULTURE; MUSHROOMS; DAMAGE; WATER;

D O I：

10.1016/j.fct.2012.05.003

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

Response surface methodology was employed to optimize the medium compositions for the production of mycelial polysaccharide from Pholiota dinghuensis Bi (PDP). As a result, the optimal medium for crude PDP production was determined as follows (g/L): glucose 36.0, corn flour 11.8, peptone 3.0, yeast extract 5.4, KH2PO4 1.0, and MgSO4 1.5. In a verification experiment, a yield of 756 +/- 38 mg/L crude PDP was obtained. Furthermore, biochemical assay and histopathological analysis showed that crude PDP exerted significant hepatoprotective effect in a dose-dependent manner against carbon tetrachloride (CCl4)-induced acute liver injury. Crude PDP prevented the increase of activities of serum alanine aminotransferase and aspartate aminotransferase, reduced the formation of malondialdehyde and enhanced the activities of superoxide dismutase and glutathione peroxidase in CCl4-induced hepatotoxicity in mice. (C) 2012 Elsevier Ltd. All rights reserved.

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收藏

页码：2681 / 2688

页数：8

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