Determination of bovine serum albumin by a resonance light-scattering technique with the mixed-complex La(Phth)(phen)3+

被引：14

作者：

Li, L ^{[1
]}

Song, GW ^{[1
]}

Fang, GR ^{[1
]}

机构：

[1] Hubei Univ, Fac Chem & Mat Sci, Wuhan 430062, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2006年 / 40卷 / 05期

关键词：

mixed-complex; resonance light-scattering; BSA; aggregation;

D O I：

10.1016/j.jpba.2005.09.004

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A mixed-complex of La(Phth)(phen)(3+) was synthesized. Resonance light-scattering characteristics of interaction between La(Phth)(phen)(3+) with bovine serum albumin (BSA) were studied. When BSA was added, aggregation of La(Phth)(phen)(3+) on the molecular surface of bovine serum albumin occurred in the pH 5.5-6.3, resulted in an enhanced resonance light-scattering (RLS) peak at 360 nm. The intensity of resonance light-scattering was found to be proportional to the concentration of BSA. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：1198 / 1201

页数：4

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