The Arabidopsis COP9 Signalosome Subunit 7 Is a Model PCI Domain Protein with Subdomains Involved in COP9 Signalosome Assembly

被引:55
作者
Dessau, Moshe [1 ]
Halimi, Yair [2 ]
Erez, Tamir [2 ]
Chomsky-Hecht, Orna [1 ]
Chamovitz, Daniel A. [2 ]
Hirsch, Joel A. [1 ]
机构
[1] Tel Aviv Univ, Fac Life Sci, Daniella Rich Inst Struct Biol, Dept Biochem, IL-69978 Ramat Aviv, Israel
[2] Tel Aviv Univ, Fac Life Sci, Dept Plant Sci, IL-69978 Ramat Aviv, Israel
基金
以色列科学基金会;
关键词
D O I
10.1105/tpc.107.053801
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The COP9 Signalosome (CSN) is a multiprotein complex that was originally identified in Arabidopsis thaliana as a negative regulator of photomorphogenesis and subsequently shown to be a general eukaryotic regulator of developmental signaling. The CSN plays various roles, but it has been most often implicated in regulating protein degradation pathways. Six of eight CSN subunits bear a sequence motif called PCI. Here, we report studies of subunit 7 (CSN7) from Arabidopsis, which contains such a motif. Our in vitro and structural results, based on 1.5 angstrom crystallographic data, enable a definition of a PCI domain, built from helical bundle and winged helix subdomains. Using functional binding assays, we demonstrate that the PCI domain (residues 1 to 169) interacts with two other PCI proteins, CSN8 and CSN1. CSN7 interactions with CSN8 use both PCI subdomains. Furthermore, we show that a C-terminal tail outside of this PCI domain is responsible for association with the non-PCI subunit, CSN6. In vivo studies of transgenic plants revealed that the overexpressed CSN7 PCI domain does not assemble into the CSN, nor can it complement a null mutation of CSN7. However, a CSN7 clone that contains the PCI domain plus part of the CSN6 binding domain can complement the null mutation in terms of seedling viability and photomorphogenesis. These transgenic plants, though, are defective in adult growth, suggesting that the CSN7 C-terminal tail plays additional functional roles. Together, the findings have implications for CSN assembly and function, highlighting necessary interactions between subunits.
引用
收藏
页码:2815 / 2834
页数:20
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