An evolving view of the eukaryotic oligosaccharyltransferase

被引:402
作者
Kelleher, DJ [1 ]
Gilmore, R [1 ]
机构
[1] Univ Massachusetts, Sch Med, Dept Mol Pharmacol & Biochem, Worcester, MA 01605 USA
关键词
dolichol; endoplasmic reticulum; N-linked oligosaccharide; oligosaccharyltransferase;
D O I
10.1093/glycob/cwj066
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Asparagine-linked glycosylation (ALG) is one of the most common protein modification reactions in eukaryotic cells, as many proteins that are translocated across or integrated into the rough endoplasmic reticulum (RER) carry N-linked oligosaccharides. Although the primary focus of this review will be the structure and function of the eukaryotic oligosaccharyltransferase (OST), key findings provided by the analysis of the archaebacterial and eubacterial OST homologues will be reviewed, particularly those that provide insight into the recognition of donor and acceptor substrates. Selection of the fully assembled donor substrate will be considered in the context of the family of human diseases known as congenital disorders of glycosylation (CDG). The yeast and vertebrate OST are surprisingly complex hetero-oligomeric proteins consisting of seven or eight subunits (Ost1p, Ost2p, Ost3p/Ost6p, Ost4p, Ost5p, Stt3p, Wbp1p, and Swp1p in yeast; ribophorin I, DAD1, N33/IAP, OST4, STT3A/STT3B, Ost48, and ribophorin II in mammals). Recent findings from several laboratories have provided overwhelming evidence that the STT3 subunit is critical for catalytic activity. Here, we will consider the evolution and assembly of the eukaryotic OST in light of recent genomic evidence concerning the subunit composition of the enzyme in diverse eukaryotes.
引用
收藏
页码:47R / 62R
页数:16
相关论文
共 155 条
[71]   Topology models for 37 Saccharomyces cerevisiae membrane proteins based on C-terminal reporter fusions and predictions [J].
Kim, H ;
Melén, K ;
von Heijne, G .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (12) :10208-10213
[72]   Determination of the membrane topology of Ost4p and its subunit interactions in the oligosaccharyl-transferase complex in Saccharomyces cerevisiae [J].
Kim, H ;
Yan, Q ;
von Heijne, G ;
Caputo, GA ;
Lennarz, WJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (13) :7460-7464
[73]   Studies on the role of the hydrophobic domain of Ost4p in interactions with other subunits of yeast oligosaccharyl transferase [J].
Kim, H ;
Park, H ;
Montalvo, L ;
Lennarz, WJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (04) :1516-1520
[74]   THE N-OLIGOSACCHARYLTRANSFERASE COMPLEX FROM YEAST [J].
KNAUER, R ;
LEHLE, L .
FEBS LETTERS, 1994, 344 (01) :83-86
[75]   The oligosaccharyltransferase complex from Saccharomyces cerevisiae -: Isolation of the OST6 gene, its synthetic interaction with OST3, and analysis of the native complex [J].
Knauer, R ;
Lehle, L .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (24) :17249-17256
[76]   Carbohydrate deficient glycoprotein syndrome type IV:: deficiency of dolichyl-P-Man:Man5GlcNAc2-PP-dolichyl mannosyltransferase [J].
Körner, C ;
Knauer, R ;
Stephani, U ;
Marquardt, T ;
Lehle, L ;
von Figura, K .
EMBO JOURNAL, 1999, 18 (23) :6816-6822
[77]   A mutation in the human MPDU1 gene causes congenital disorder of glycosylation type If (CDG-If) [J].
Kranz, C ;
Denecke, J ;
Lehrman, MA ;
Ray, S ;
Kienz, P ;
Kreissel, G ;
Sagi, D ;
Peter-Katalinic, J ;
Freeze, HH ;
Schmid, T ;
Jackowski-Dohrmann, S ;
Harms, E ;
Marquardt, T .
JOURNAL OF CLINICAL INVESTIGATION, 2001, 108 (11) :1613-1619
[78]   CHARACTERIZATION OF RIBOSOMAL BINDING-SITE IN RAT-LIVER ROUGH MICROSOMES - RIBOPHORINS-I AND RIBOPHORINS-II, 2 INTEGRAL MEMBRANE PROTEINS RELATED TO RIBOSOME BINDING [J].
KREIBICH, G ;
CZAKOGRAHAM, M ;
GREBENAU, R ;
MOK, W ;
RODRIGUEZBOULAN, E ;
SABATINI, DD .
JOURNAL OF SUPRAMOLECULAR STRUCTURE, 1978, 8 (03) :279-302
[79]   Isolation and characterization of dolichol-linked oligosaccharides from Haloferax volcanii [J].
Kuntz, C ;
Sonnenbichler, J ;
Sonnenbichler, I ;
Sumper, M ;
Zeitler, R .
GLYCOBIOLOGY, 1997, 7 (07) :897-904
[80]   STRUCTURE AND BIOSYNTHESIS OF PROKARYOTIC GLYCOPROTEINS [J].
LECHNER, J ;
WIELAND, F .
ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :173-194