An invertebrate defense molecule activates membrane conductance in mammalian cells by means of its lectin-like domain

被引：12

作者：

Bloc, A

Lucas, R ^{[1
]}

Van Dijck, E

Bilej, M

Dunant, Y

De Baetselier, P

Beschin, A

机构：

[1] Ctr Med Univ Geneva, Fac Med, Dept Pharmacol, Geneva 4, Switzerland

[2] Univ Konstanz, Dept Biochem Pharmacol, D-78457 Constance, Germany

[3] Free Univ Brussels VIB, Dept Immunol Parasitol & Ultrastruct, B-1640 Rhode St Genese, Belgium

[4] Acad Sci Czech Republ, Inst Microbiol, Dept Immunol, Prague 4, Czech Republic

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2002年 / 26卷 / 01期

关键词：

CCF-1; TNF; amiloride-sensitive conductance; innate immunity; lectin;

D O I：

10.1016/S0145-305X(01)00054-4

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The invertebrate defense molecule Coelomic Cytolytic Factor-1 (CCF-1) and the mammalian cytokine Tumor Necrosis Factor (TNF) share a similar lectin-like domain that, upon interaction with specific sugars, causes lysis of African trypanosomes. In contrast to TNF, CCF- I does not require an acidification of a lysosomal compartment for this activity. Moreover, we could demonstrate using the whole cell patch clamp technique that both TNF and CCF-1 activate amiloride-sensitive channels in mammalian cells, in a TNF receptor-independent way, but, unlike TNF, CCF-1 does not require acidic conditions for this activity. These data confirm the functional analogies of an invertebrate defense molecule and a mammalian cytokine, based on a similar lectin-like interaction. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：35 / 43

页数：9

共 21 条

[1] Identification and cloning of a glucan- and liopoplysaccharide-binding protein from Eisenia foetida earthworm involved in the activation of prophenoloxidase cascade [J].