Applications of quantum dots as probes in immunosensing of small-sized analytes

被引:163
作者
Esteve-Turrillas, Francesc A. [1 ]
Abad-Fuentes, Antonio [1 ]
机构
[1] IATA CSIC, Dept Biotechnol, Valencia 46980, Spain
关键词
Antibody; Conjugation; Hapten; Immunoassay; ELISA; Fluorescence; FRET; Immunosensor; Quantum dot; RESONANCE ENERGY-TRANSFER; SENSITIVE DETECTION; ELECTROGENERATED CHEMILUMINESCENCE; PHOTOELECTROCHEMICAL IMMUNOASSAY; COMPETITIVE FLUOROIMMUNOASSAY; MULTIPLEXED IMMUNOASSAYS; ULTRASENSITIVE DETECTION; FLUORESCENT IMMUNOASSAY; AMPHIPHILIC POLYMER; SILICA NANOSPHERE;
D O I
10.1016/j.bios.2012.09.025
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Quantum dots (QDs) are semiconductor nanoparticles with very interesting optical properties, like high quantum yield or narrow and size-tuneable fluorescence spectra. Current applications of QDs are widespread, their use as fluorescence labels in bioassays being one of the most promising. These nanoparticles are usually conjugated to highly specific biomolecules like antibodies, oligonucleotides, enzymes or aptamers to improve assay selectivity. In this review, QD surface passivation, conjugation to biomolecules, and purification strategies are discussed with special emphasis to the development of QD-based immunoassays for the detection of low molecular weight compounds given the relevance of this sort of analytes in health, food safety, pharmaceutical, or environmental monitoring areas. The aim of this review is to summarise the main achievements attained so far and to initialise researchers in the field of antibody-based assays employing QDs as labels, such as fluorescence-linked immunosorbent assay (FLISA), fluorescence (or Forster) resonance energy transfer (FRET), immunochromatographic methods, and immunosensors. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:12 / 29
页数:18
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