Spin-state-selective TPPI:: A new method for suppression of heteronuclear coupling constants in multidimensional NMR experiments

被引:7
作者
Schulte-Herbrüggen, T
Briand, J
Meissner, A
Sorensen, OW
机构
[1] Carlsberg Lab, Dept Chem, DK-2500 Valby, Denmark
[2] SmithKline Beecham Pharmaceut, Dept Phys & Struct Chem, King Of Prussia, PA 19406 USA
关键词
multidimensional NMR; S-3; TPPI; HSQC; TROSY;
D O I
10.1006/jmre.1999.1787
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A novel multidimensional NMR pulse sequence tool, spin-state-selective time-proportional phase incrementation (S-3 TPPI), is introduced. It amounts to application of different TPPIs on the two components of doublets so that their frequencies can be manipulated independently, The chief application is for suppression of large heteronuclear one-bond coupling constants in indirect dimensions of multidimensional experiments without interchanging the two transverse magnetization components of doublets as conventional decoupling does, which is advantageous when they relax at different rates such as by partial compensation of dipolar and CSA relaxation contributions. For experimental confirmation we use a sample of N-15-labeled neural cell adhesion molecule modules 1 and 2, a protein with a molecular weight of about 20 kDa, The new tool is general and can be combined with many multidimensional NMR experiments for proteins. (C) 1999 Academic Press.
引用
收藏
页码:443 / 446
页数:4
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