Evaluation of the trophic effect of human placental polydeoxyribonucleotide on human knee skin fibroblasts in primary culture

被引：28

作者：

Muratore, O ^{[1
]}

Schito, AP ^{[1
]}

Cattarini, G ^{[1
]}

Tonoli, EL ^{[1
]}

Gianoglio, S ^{[1
]}

Schiappacasse, S ^{[1
]}

Felli, L ^{[1
]}

Picchetta, F ^{[1
]}

Schito, GC ^{[1
]}

机构：

[1] UNIV GENOA, CHAIR ORTHOPAED & TRAUMATOL, I-16132 GENOA, ITALY

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 1997年 / 53卷 / 03期

关键词：

human placental polydeoxyribonucleotide (PDRN); primary tissue culture; human skin fibroblasts; wound healing;

D O I：

10.1007/PL00000605

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple assay capable of evaluating the trophic effect of growth factors or active principles on human skill diploid fibroblasts in primary culture has been developed. The results indicate that at physiological concentrations (20-100 mu g/ml) a human placental polydeoxyribonucleotide (PDRN) preparation enhances the growth of human skin diploid fibroblasts of the knee in primary culture. This effect is consistently reproducible in the case of patients over 60 years of age, and may explain previously reported data on the successful clinical applications of human placental preparations, suggesting a selective benefit of PDRN in wound healing when compared to other treatments.

引用

页码：279 / 285

页数：7