Kinase-catalyzed modification of gold nanoparticles:: A new approach to colorimetric kinase activity screening

被引：243

作者：

Wang, ZX

Lévy, R

Fernig, DG

Brust, M ^{[1
]}

机构：

[1] Univ Liverpool, Dept Chem, Ctr Nanoscale Sci, Liverpool L69 7ZD, Merseyside, England

[2] Univ Liverpool, Sch Biol Sci, Liverpool L69 7ZD, Merseyside, England

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 07期

关键词：

D O I：

10.1021/ja058135y

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Peptide-stabilized gold nanoparticles have been enzymatically biotinylated by a kinase-catalyzed reaction using biotin-ATP as a cosubstrate. Upon mixing with avidin-modified particles, solutions of biotinylated particles change color from red to blue, indicating aggregation of particles. On the basis of this reaction, we have developed a simple colorimetric test to monitor kinase inhibitor activity. Copyright © 2006 American Chemical Society.

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页码：2214 / 2215

页数：2

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