Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum

被引：227

作者：

Laursen, Tomas ^{[1
,2
,3
,4
,5
]}

Borch, Jonas ^{[2
,6
]}

Knudsen, Camilla ^{[1
,2
,3
,4
]}

Bavishi, Krutika ^{[1
,2
,3
,4
]}

Torta, Federico ^{[7
]}

Martens, Helle Juel ^{[4
]}

Silvestro, Daniele ^{[4
]}

Hatzakis, Nikos S. ^{[2
,8
]}

Wenk, Markus R. ^{[9
]}

Dafforn, Timothy R. ^{[10
,11
]}

Olsen, Carl Erik ^{[1
,2
]}

Motawia, Mohammed Saddik ^{[1
,2
,3
,4
]}

Hamberger, Bjorn ^{[1
,2
]}

Moller, Birger Lindberg ^{[1
,2
,3
,4
,12
]}

Bassard, Jean-Etienne ^{[1
,2
,3
,4
]}

机构：

[1] Univ Copenhagen, Dept Plant & Environm Sci, Plant Biochem Lab, DK-1871 Frederiksberg C, Denmark

[2] BioSYNergy, Ctr Synthet Biol, DK-1871 Frederiksberg C, Denmark

[3] VILLUM Res Ctr Plant Plast, DK-1871 Frederiksberg C, Denmark

[4] Univ Copenhagen, Copenhagen Plant Sci Ctr, DK-1871 Frederiksberg C, Denmark

[5] Joint BioEnergy Inst, Feedstocks Div, Emeryville, CA 94608 USA

[6] Univ Southern Denmark, Dept Biochem & Mol Biol, VILLUM Ctr Bioanalyt Sci, DK-5230 Odense M, Denmark

[7] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Biochem, Singapore 117597, Singapore

[8] Univ Copenhagen, Nanosci Ctr, Dept Chem, DK-2100 Copenhagen, Denmark

[9] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Biol Sci, Singapore 117597, Singapore

[10] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

[11] Her Majestys Govt, Dept Business Energy & Ind Strategy, London, England

[12] Carlsberg Res Lab, DK-1799 Copenhagen V, Denmark

来源：

SCIENCE | 2016年 / 354卷 / 6314期

基金：

新加坡国家研究基金会; 欧洲研究理事会;

关键词：

CYANOGENIC GLUCOSIDE DHURRIN; BICOLOR L MOENCH; BIOSYNTHESIS; PLASTICITY; PATHWAYS; PROTEIN;

D O I：

10.1126/science.aag2347

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired metabolic cross-talk while maintaining metabolic flexibility. Here we report the isolation of the dynamic metabolon that catalyzes the formation of the cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The metabolon was reconstituted in liposomes, which demonstrated the importance of membrane surface charge and the presence of the glucosyltransferase for metabolic channeling. We used in planta fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to study functional and structural characteristics of the metabolon. Understanding the regulation of biosynthetic metabolons offers opportunities to optimize synthetic biology approaches for efficient production of high-value products in heterologous hosts.

引用

页码：890 / 893

页数：4

共 16 条

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Reversible compartmentalization of de novo purine biosynthetic complexes in living cells [J].