A surface-scanning coil detector for real-time, in-situ detection of bacteria on fresh food surfaces

被引：64

作者：

Chai, Yating ^{[1
]}

Horikawa, Shin ^{[1
]}

Li, Suiqiong ^{[1
]}

Wikle, Howard C. ^{[1
]}

Chin, Bryan A. ^{[1
]}

机构：

[1] Auburn Univ, Mat Res & Educ Ctr, Auburn, AL 36849 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2013年 / 50卷

关键词：

Surface-scanning coil detector; Magnetoelastic biosensor; Real-time detection; In-situ detection; Salmonella typhimurium; Fresh food surfaces; SALMONELLA-TYPHIMURIUM;

D O I：

10.1016/j.bios.2013.06.056

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 [生物物理学];

摘要：

Proof-in-principle of a new surface-scanning coil detector has been demonstrated. This new coil detector excites and measures the resonant frequency of free-standing magnetoelastic (ME) biosensors that may now be placed outside the coil boundaries. With this coil design, the biosensors are no longer required to be placed inside the coil before frequency measurement. Hence, this new coil enables bacterial pathogens to be detected on fresh food surfaces in real-time and in-situ. The new coil measurement technique was demonstrated using an E2 phage-coated ME biosensor to detect Salmonella typhimurium on tomato surfaces. Real-time, in-situ detection was achieved with a limit of detection (LOD) statistically determined to be lower than 1.5 x 10(3) CFU/mm(2) with a confidence level of difference higher than 95% (p < 0.05). (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：311 / 317

页数：7

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