Binding interaction of gatifloxacin with bovine serum albumin

被引：121

作者：

Guo, M ^{[1
]}

Zou, JW ^{[1
]}

Yi, PG ^{[1
]}

Shang, ZC ^{[1
]}

Hu, GX ^{[1
]}

Yu, QS ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Chem, Hangzhou 310027, Peoples R China

来源：

ANALYTICAL SCIENCES | 2004年 / 20卷 / 03期

关键词：

D O I：

10.2116/analsci.20.465

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The binding of gatifloxacin to bovine serum albumin (BSA) in aqueous solution was studied using fluorescence spectroscopy and absorbance spectra. Further, the interactions influenced by Fe3+ and Cu2+ were also explored in this work. Based on Scatchard's site-binding model and florescence quenching, practical formulas for small molecule ligands to bio-macromolecules have been proposed. The binding parameters were measured according to suggested models, and the binding distance and the transfer efficiency of energy between gatifloxacin and BSA were also obtained in view of the Forster theory of non-radiation energy transfer. The effect of gatifloxacin on the conformation of BSA has also been analyzed using synchronous fluorescence spectroscopy.

引用

页码：465 / 470

页数：6

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